Human Bone Sialoprotein ELISA Kit
(ab277706)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 9 pg/ml
- Range: 21.88 pg/ml - 1400 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
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Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.2% Inter-assay Sample n Mean SD CV% Serum 3 7.3% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
9 pg/ml -
Range
21.88 pg/ml - 1400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 106 104% - 107% Hep Plasma 114 106% - 119% EDTA Plasma 104 89% - 114% Cit plasma 99 85% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Bone Sialoprotein ELISA kit (ab277706) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Bone Sialoprotein in human serum and plsma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Bone Sialoprotein with 9 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Bone Sialoprotein, also known as IBSP, is a matrix protein that plays a role in mineralization of bone and other calcified structures. The exact function of this protein is currently under investigation. The immunogen with which the antibodies in this kit were raised is composed of the full-length mature protein. This protein is not homologous with other species; as such, it is not expected to cross-react.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Bone Sialoprotein (IBSP) Capture Antibody 1 x 600µl 10X Human Bone Sialoprotein (IBSP) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Bone Sialoprotein (IBSP) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment. -
Post-translational
modificationsN-glycosylated; glycans consist of sialated and core-fucosylated bi-, tri- and tetraantennary chains.
O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.
Sulfated on either Tyr-313 or Tyr-314. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- BNSP
- Bone sialoprotein 2
- Bone sialoprotein II
see all -
Database links
- Entrez Gene: 3381 Human
- Omim: 147563 Human
- SwissProt: P21815 Human
- Unigene: 518726 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Bone Sialoprotein standard curve in Sample Diluent NS.The Bone Sialoprotein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Bone Sialoprotein in human serum and plasma samples.The concentrations of Bone Sialoprotein were measured in duplicates, interpolated from the Bone Sialoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Bone Sialoprotein concentration was determined to be 471 pg/mL in serum, 1,397 pg/mL in plasma (citrate) and 647 pg/mL in plasma (heparin) and 434 pg/mL in plasma (EDTA).
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Bone Sialoprotein concentration was determined to be 630 pg/mL with a range of 218 – 1,437 pg/mL.
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Recombinant Antibody BenefitsTo learn more about the advantages of recombinant antibodies see here.