Astana Biomed Group, an authorized Abcam distributor in Central Asia

PE Anti-PD-L1 antibody [28-8] (ab209962)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [28-8] to PD-L1
  • Suitable for: Flow Cyt
  • Reacts with: Chinese hamster
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-PD-L1 antibody [28-8]
    See all PD-L1 primary antibodies

  • Description

    PE Rabbit monoclonal [28-8] to PD-L1

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Chinese hamster
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human PD-L1 (extracellular). The immunogen contains the specific extracellular domain of huPD-L1 (Phe19-Thr239). See reference for more info - www.ncbi.nlm.nih.gov/pmc/articles/PMC4561627/
    Database link: Q9NZQ7

  • Positive control

    • Flow Cyt: CHO-PDL1 cells

  • General notes

    Anti-PD-L1 antibody [28-8] has been used as detector antibody in Human PD-L1 SimpleStep ELISA® kit (ab214565)

    Additional information on positive controls:

    Cell Lines:
    Positives: B-CPAP- high, ES-2- medium, HCC70 - low

    For primary negative control, isotype control, RabMAb negative control antibody (ab172730) is recommended.

    For negative control sample, cell line COLO205 is recommended.

    For PD-L1 protein, see ab167713.

    Recommended protocols:

    For recommended Flow Cytometry (Flow Cyt) protocol, please refer to the protocol book (line 1) in the protocol section and/or here (downloadable copy) 

    Alternative versions available:
    Anti-PD-L1 antibody [28-8] (ab205921) - Knockout validated

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - PE Anti-PD-L1 antibody [28-8] (ab209962)
    Flow Cytometry - PE Anti-PD-L1 antibody [28-8] (ab209962)

    Overlay histgram showing CHO (blue line) and CHO-PD-L1 transfected (red line) cells stained with ab209962.

    The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209962, 1/100 dilution) for 30 min at 4ºC.

    Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/42 bandpass filter.

  • PE Anti-PD-L1 antibody [28-8] (ab209962)
    PE Anti-PD-L1 antibody [28-8] (ab209962)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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