pCambia1200 Plant Expression Vector (ab275778)
Overview
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Product name
pCambia1200 Plant Expression Vector -
General notes
Vector contains hygromycin B resistance for plant selection, chloramphenicol resistance for bacterial selection and no reporter gene.
The pCambia1200 vector offers:
High copy number in E.coli for high DNA yields
pVS1 replicon for high stability in Agrobacterium
Small size
Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with chloramphenicol
Plant selection with hygromycin BPlant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV35S promoter and terminated by the CaMV35S polyA signal.
This vector contains minimal heterologous sequences for plant transformation and selection of transformants, allowing insertion of desired genes for transformation into plants but require all promoter and terminator sequences for plant expression of newly cloned genes. The pUC18 polylinker within the lacZa fragment allows blue/white screening of clones in E. coli cloning work.
Concentration of Antibiotics for selection.
In bacterial system:
Chloramphenicol resistance (cat – chloramphenicol acetyltransferase from E.coli Tn-cam204) in bacteria: use 10μg/mL in E.coli and A.tumefaciens.In plant system:
Resistance Gene hph (From a Klebsiella species, via E.coli) Transformed plant selection antibiotic Hygromycin B Concentration in Arabidopsis 50 μg/mL in germination medium Concentration in Rice 50 μg/mL in 2N6 then in RGH6 Concentration in Tobacco 50 μg/mL in RMOP then in MST
Properties
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Form
Lyophilized -
Storage instructions
Shipped at 4°C. Store at -20°C. -
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Images
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pCambia1200 Plant Expression Vector (ab275778)