Total Carbohydrate Assay Kit - Quantification (ab155891)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Adherent cells, Suspension cells, Tissue, Tissue Extracts
Overview
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Product name
Total Carbohydrate Assay Kit - Quantification -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells, Tissue Extracts -
Assay type
Quantitative -
Assay time
0h 30m -
Product overview
Total Carbohydrate Assay (ab155891) is a simple, sensitive and robust method of detecting virtually all carbohydrates.
The carbohydrate assay protocol is based on the phenol - sulfuric acid assay. In the assay, polysaccharides (mono, di, tri, etc.) and their derivatives, in the presence of sulfuric acid, are hydrolyzed to monomers and converted to furfural or hydroxyfurfural. These react with the developer to form a chromogen that can be quantified by measuring the absorbance at λ = 490 nm.
The total carbohydrate assay kit can detect most forms of carbohydrates, including simple and complex saccharides, glycans, glycoproteins and glycolipids.
Total carbohydrate assay protocol summary:
- add samples and glucose standards to wells
- add concentrated sulfuric acid and incubate for 15 min at 90ºC
- add developer and incubate for 5 min
- analyze with a microplate reader -
Notes
Carbohydrates play important structural as well as chemical roles in all living systems. Detection of total carbohydrates, therefore, has wide applications.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 100 tests Carbohydrate Assay Buffer WM 1 x 25ml Developer Brown Bottle 1 x 3ml D-Glucose Standard Yellow 1 x 0.2ml -
Research areas
Images
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Functional assays: Carbohydrates (ab155891)
Carbohydrates in food items and mouse tissue lysates, shown as mg per mg of extracted protein (duplicates; +/- SD).
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Functional assays: Carbohydrates (ab155891)Carbohydrates measured in orange juice and caster sugar. Background signal subtracted, duplicates; +/- SD.
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Functional assays: Carbohydrates (ab155891) -
D-Glucose standard curveD-Glucose standard curve generated using the method described in the protocol bookelt.
