NSC 146109 hydrochloride, Cell-permeable p53 activator (ab142144)
Key features and details
- Cell-permeable p53 activator
- CAS Number: 59474-01-0
- Purity: > 98%
- Soluble in DMSO to 100 mM and in ethanol to 10 mM
- Form / State: Solid
- Source: Synthetic
Overview
-
Product name
NSC 146109 hydrochloride, Cell-permeable p53 activator -
Description
Cell-permeable p53 activator -
Biological description
Cell-permeable p53 activator. Genotype-selective antitumor agent. Active in vitro. -
Purity
> 98% -
CAS Number
59474-01-0 -
Chemical structure
Properties
-
Chemical name
(10-Methylanthracen-9-yl)methyl carbamimidothioate hydrochloride -
Molecular weight
316.85 -
Molecular formula
C17H16N2S.HCl -
PubChem identifier
16759161 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM and in ethanol to 10 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
SMILES
CC1=C2C=CC=CC2=C(C3=CC=CC=C13)CSC(=N)N.Cl -
Source
Synthetic
-
Research areas
Images
-
Functional Studies - NSC 146109 hydrochloride, Cell-permeable p53 activator (ab142144)ab26 staining p53 in U20S cells treated with NSC 146109 hydrochloride (ab142144), by ICC/IF. Increase in p53 expression correlates with increased concentration of NSC 146109 hydrochloride, as described in literature.
The cells were incubated at 37°C for 5 hour in media containing different concentrations of ab142144 (NSC 146109 hydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab26 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.