Nitric Oxide Assay Kit (ab272517)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Extracts, Urine, Whole Blood
Overview
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Product name
Nitric Oxide Assay Kit
See all Nitric oxide kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Saliva, Urine, Serum, Plasma, Whole Blood, Tissue Extracts, Cell Lysate -
Assay type
Quantitative -
Range
0.6 µM - 200 µM -
Product overview
Nitric Oxide Assay Kit (ab272517) is a simple, direct and automation-ready procedure for measuring NO. Since NO is oxidized to nitrite and nitrate, it is common practice to quantitate total NO2 - /NO3 - as a measure for NO level. This Nitric Oxide Assay Kit is designed to accurately measure NO production following reduction of nitrate to nitrite using an improved Griess method. The procedure is simple and the time required for sample pre-treatment and assay is reduced to as little as 30 minutes.
Sensitive and accurate: Detection range 0.6 - 200 µM in 96-well plate.
Rapid and reliable: Using an optimized VCl3 reagent, the time required for reduction of NO3 – to NO2 - is 10 min at 60°C.
Simple and high-throughput: The procedure involves mixing sample with three reagents, incubation for 10 minutes at 60°C and reading the optical density. Can be readily automated to measure thousands of samples per day.
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Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests NaOH 1 x 1ml Nitrite Standard (1 mM) 1 x 1ml Reagent A 1 x 12ml Reagent B 1 x 500µl Reagent C 1 x 12ml ZnSO4 1 x 1ml -
Research areas
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Relevance
Nitric oxide (NO) is a key vertebrate biological messenger, playing an important role in neurotransmission, vascular regulation, immune responses and apoptosis. NO , also known as "endothelium-derived relaxing factor" or "EDRF", is synthesized from L-arginine, oxygen and NADPH by various NO synthases. Most of the NO in the cell is oxidized to nitrite (NO2-) and nitrate (NO3-), and therefore the concentrations of these anions are generally as a quantitative measure of NO production.
Images
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Nitric Oxide Assay Kit Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
