GST Assay Kit (Colorimetric) (ab65326)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 4 mU/well
Overview
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Product name
GST Assay Kit (Colorimetric)
See all GST kits -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate -
Assay type
Enzyme activity -
Sensitivity
Assay time
1h 30mSpecies reactivity
Reacts with: Mammals, Other speciesProduct overview
GST Assay Kit (Colorimetric) (ab65326) is used to detect GST activity in crude cell lysates and purified protein fractions, and also to quantitate GST-tagged fusion proteins.
The GST assay protocol is based on the GST-catalyzed reaction between GSH and the GST substrate CDNB.
CDNB has the broadest range of isozyme detectability (e.g., alpha-, mu-, pi-, and other GST isoforms). The GST-catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected by spectrophotometer at 340 nm.
One unit of GST activity is defined as the amount of enzyme producing 1 µmol of GS-DNB conjugate/min under the conditions of the assay. Detection limit of assay: active GST
GST assay protocol summary:
- add samples and positive control to wells
- add glutathione to wells
- add reaction mix
- analyze with a microplate reader for at least 10 minNotes
Glutathione S-transferase (GST, EC 2.5.1.13) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds.
Platform
Microplate readerProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Glutathione (GSH, lyophilized) Yellow 2 x 17mg GST Assay Buffer WM 1 x 25ml GST Positive Control (0.625 µg/µl) Green 1 x 10µl GST Substrate (CDNB) Red 1 x 0.1ml -
Research areas
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Relevance
Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. -
Cellular localization
Cytoplasmic -
Alternative names
- Glutathione S transferase A1
- Glutathione S transferase A2
- GST class alpha 1
see all
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Glutathione (GSH, lyophilized) Yellow 2 x 17mg GST Assay Buffer WM 1 x 25ml GST Positive Control (0.625 µg/µl) Green 1 x 10µl GST Substrate (CDNB) Red 1 x 0.1ml -
Research areas
-
Relevance
Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. -
Cellular localization
Cytoplasmic -
Alternative names
- Glutathione S transferase A1
- Glutathione S transferase A2
- GST class alpha 1
see all
Images
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Functional Studies - GST Activity Assay Kit (Colorimetric) (ab65326)
GST Kinetic Assay performed according to the protocol.
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Functional Studies - GST Activity Assay Kit (Colorimetric) (ab65326)GST activity calculated in cell lysates, with background signal subtracted (duplicates +/- SD), using equation in protocol.
