GST Activity Assay Kit (Fluorometric) (ab65325)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Other biological fluids, Plasma, Purified protein, Saliva, Serum, Tissue Lysate, Urine
- Sensitivity: 0.5 mU/well
Overview
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Product name
GST Activity Assay Kit (Fluorometric)
See all GST kits -
Detection method
Fluorescent -
Sample type
Saliva, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Purified protein, Tissue Lysate -
Assay type
Enzyme activity -
Sensitivity
Assay time
2h 00mProduct overview
Abcam's GST Activity Assay Kit (Fluorometric) provides a simple, fluorescence-based in vitro assay for detecting the GST activity using fluorescence plate reader. The assay utilizes monochlorobimane (MCB), a dye that reacts with glutathione. The free form of MCB is almost nonfluorescent, whereas the dye fluoresces in blue (Ex/Em = 380/461 nm) after reaction with glutathione catalysed by GST. The change in fluorescence overtime allows for an easy measurement of the sample GST level.
The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein.
Visit our FAQs page for tips and troubleshooting.Notes
Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds.
Platform
Microplate readerProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Glutathione (lyophilized) Yellow 2 vials GST Assay Buffer WM 1 x 25ml GST Standard (5 mU/µl) Green 1 x 10µl Monochlorobimane Substrate Red 1 x 200µl -
Research areas
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Relevance
Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. -
Cellular localization
Cytoplasmic -
Alternative names
- Glutathione S transferase A1
- Glutathione S transferase A2
- GST class alpha 1
see all
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Glutathione (lyophilized) Yellow 2 vials GST Assay Buffer WM 1 x 25ml GST Standard (5 mU/µl) Green 1 x 10µl Monochlorobimane Substrate Red 1 x 200µl -
Research areas
-
Relevance
Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. -
Cellular localization
Cytoplasmic -
Alternative names
- Glutathione S transferase A1
- Glutathione S transferase A2
- GST class alpha 1
see all
Images
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Functional Studies - GST Activity Assay Kit (Fluorometric) (ab65325)Standard Calibration Curve of GST Measured by Fluorometry. Various amounts of standard GST were incubated with GSH and MCB according to the kit instructions. Fluorescence was measured at ex/em 380/460 nm.
