Myeloperoxidase (MPO) Peroxidation Activity Assay Kit (Fluorometric) (ab273334)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Myeloperoxidase (MPO) Peroxidation Activity Assay Kit (Fluorometric)
See all Myeloperoxidase kits -
Detection method
Fluorescent -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
Myeloperoxidase (MPO) Peroxidation Activity Assay Kit (Fluorometric) (ab273334) oxidizes a substrate to generate fluorescence (Ex/Em = 535/587 nm), which is directly proportional to total peroxidase activity in the sample. A specific MPO inhibitor is provided, which suppresses peroxidase activity due to MPO. This permits differentiation of MPO-mediated peroxidation from the activity of other peroxidases which may be present in the samples.
This kit provides a sensitive, quick and easy method for detecting MPO activity and can detect less than 2 µU of MPO activity. -
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Hydrogen Peroxide 1 x 50µl MPO Assay Buffer 1 x 50ml MPO Inhibitor 1 x 100µl MPO Peroxidation Substrate 1 x 200µl MPO Positive Control Lyophilized 1 vial Resorufin Standard 1 x 50µl -
Research areas
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Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
Involvement in disease
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
Sequence similarities
Belongs to the peroxidase family. XPO subfamily. -
Cellular localization
Lysosome. - Information by UniProt
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Alternative names
- 84 kDa myeloperoxidase
- 89 kDa myeloperoxidase
- EC 1.11.1.7
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Images
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Example data
Example data.
Resorufin Standard Curve.
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Example dataExample data.
Myeloperoxidase Activity (∆RFU +/- inhibitor) for freshly prepared white blood cell lysate (0.5µg), perfused rat spleen lysate (0.9µg), Positive Control, and reagent blank.
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Example dataExample data.
Calculated Myeloperoxidase activity in white blood cell lysate and rat spleen lysate.