Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BRN3A antibody [EP1972Y] (ab81213)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1972Y] to BRN3A
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BRN3A antibody [EP1972Y]
    See all BRN3A primary antibodies

  • Description

    Rabbit monoclonal [EP1972Y] to BRN3A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human BRN3A aa 300-400 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Human brain lysate; SW480; Mouse cerebrellum; Rat brain; Rat spinal cord. ICC/IF: HeLa cells. Flow Cyt: SH-SY5Y cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-BRN3A antibody [EP1972Y] (ab81213)
    Western blot - Anti-BRN3A antibody [EP1972Y] (ab81213)
    All lanes : Anti-BRN3A antibody [EP1972Y] (ab81213) at 1/2000 dilution (purified)

    Lane 1 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse cerebellum lysates
    Lane 3 : Rat brain lysates
    Lane 4 : Rat spinal cord lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 43 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-BRN3A antibody [EP1972Y] (ab81213)
    Flow Cytometry - Anti-BRN3A antibody [EP1972Y] (ab81213)
    Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling BRN3A with purified ab81213 at 1:100 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Western blot - Anti-BRN3A antibody [EP1972Y] (ab81213)
    Western blot - Anti-BRN3A antibody [EP1972Y] (ab81213)
    All lanes : Anti-BRN3A antibody [EP1972Y] (ab81213) (unpurified)

    Lane 1 : LNCaP
    Lane 2 : SW480
    Lane 3 : PC-3
    Lane 4 : Human brain lysate

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

  • Western blot - Anti-BRN3A antibody [EP1972Y] (ab81213)
    Western blot - Anti-BRN3A antibody [EP1972Y] (ab81213) This image is courtesy of an Anonymous Abreview.
    All lanes : Anti-BRN3A antibody [EP1972Y] (ab81213) at 1/2000 dilution (unpurified)

    Lane 1 : Adult mouse cerebellum lysate
    Lane 2 : Adult mouse lower brainstem lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated Mouse anti-Rabbit IgG monoclonal. at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa


    Exposure time: 2 minutes

    See Abreview

  • Flow Cytometry - Anti-BRN3A antibody [EP1972Y] (ab81213)
    Flow Cytometry - Anti-BRN3A antibody [EP1972Y] (ab81213)

    Overlay histogram showing SH-SY5Y cells stained with unpurified ab81213 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81213, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Anti-BRN3A antibody [EP1972Y] (ab81213)
    Anti-BRN3A antibody [EP1972Y] (ab81213)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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