Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸ 10 µl 120 614 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1972Y] to BRN3A
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-BRN3A antibody [EP1972Y]
See all BRN3A primary antibodies
Description

Rabbit monoclonal [EP1972Y] to BRN3A
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human BRN3A aa 300-400 (C terminal). The exact sequence is proprietary.
Positive control
- WB: Human brain lysate; SW480; Mouse cerebrellum; Rat brain; Rat spinal cord. ICC/IF: HeLa cells. Flow Cyt: SH-SY5Y cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1972Y
Isotype

IgG
Research areas

Western blot - Anti-BRN3A antibody [EP1972Y] (ab81213)

All lanes : Anti-BRN3A antibody [EP1972Y] (ab81213) at 1/2000 dilution (purified)

Lane 1 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse cerebellum lysates
Lane 3 : Rat brain lysates
Lane 4 : Rat spinal cord lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 43 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-BRN3A antibody [EP1972Y] (ab81213)

Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling BRN3A with purified ab81213 at 1:100 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-BRN3A antibody [EP1972Y] (ab81213)

All lanes : Anti-BRN3A antibody [EP1972Y] (ab81213) (unpurified)

Lane 1 : LNCaP
Lane 2 : SW480
Lane 3 : PC-3
Lane 4 : Human brain lysate

Predicted band size: 43 kDa
Observed band size: 43 kDa
Western blot - Anti-BRN3A antibody [EP1972Y] (ab81213) This image is courtesy of an Anonymous Abreview.

All lanes : Anti-BRN3A antibody [EP1972Y] (ab81213) at 1/2000 dilution (unpurified)

Lane 1 : Adult mouse cerebellum lysate
Lane 2 : Adult mouse lower brainstem lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP-conjugated Mouse anti-Rabbit IgG monoclonal. at 1/10000 dilution

Performed under reducing conditions.

Predicted band size: 43 kDa
Observed band size: 43 kDa

Exposure time: 2 minutes
See Abreview
Flow Cytometry - Anti-BRN3A antibody [EP1972Y] (ab81213)

Overlay histogram showing SH-SY5Y cells stained with unpurified ab81213 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81213, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
Anti-BRN3A antibody [EP1972Y] (ab81213)

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