Mouse/Rat Bcl-xL Antibody Pair - BSA and Azide free (ab244046)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse, Rat
Overview
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Product name
Mouse/Rat Bcl-xL Antibody Pair - BSA and Azide free
See all Bcl-XL kits -
Assay type
ELISA set -
Range
0.19 pg/ml - 12 pg/ml -
Species reactivity
Reacts with: Mouse, Rat -
Product overview
The Antibody Pair can be used to quantify Mouse Bcl-xL/Rat. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab227899), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Mouse/Rat Bcl-xL Capture Antibody (unconjugated) 1 x 100µg Mouse/Rat Bcl-xL Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
Isoform Bcl-X(S) promotes apoptosis. -
Tissue specificity
Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain. -
Sequence similarities
Belongs to the Bcl-2 family. -
Domain
The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death. -
Post-translational
modificationsProteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity. -
Cellular localization
Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope. - Information by UniProt
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Alternative names
- Apoptosis regulator Bcl X
- Apoptosis regulator Bcl-X
- Apoptosis regulator BclX
see all -
Database links
- Entrez Gene: 12048 Mouse
- Entrez Gene: 24888 Rat
- SwissProt: Q64373 Mouse
- SwissProt: P53563 Rat
- Unigene: 238213 Mouse
- Unigene: 10323 Rat
Images
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Sandwich ELISA - Mouse/Rat Bcl-xL Antibody Pair - BSA and Azide free (ab244046)To learn more about the advantages of recombinant antibodies see here.