Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Themis antibody [EPR7353] - BSA and Azide free (ab248177)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7353] to Themis - BSA and Azide free
  • Suitable for: ICC, IP, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Themis antibody [EPR7353] - BSA and Azide free
    See all Themis primary antibodies

  • Description

    Rabbit monoclonal [EPR7353] to Themis - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Jurkat cells; WB: Jurkat and MOLT-4 cells, Mouse and rat thymus lysate; ICC: Jurkat cells Flow cyt: Jurkat cells.

  • General notes

    Ab248177 is the carrier-free version of ab126771. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248177 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunoprecipitation - Anti-Themis antibody [EPR7353] - BSA and Azide free (ab248177)
    Immunoprecipitation - Anti-Themis antibody [EPR7353] - BSA and Azide free (ab248177)

    This data was developed using ab126771, the same antibody clone in a different buffer formulation.
    Purified ab126771 at 1/30 dilution (2µg) immunoprecipitating Themis in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab126771 + Jurkat whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126771 in Jurkat whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 73 kDa

  • Western blot - Anti-Themis antibody [EPR7353] - BSA and Azide free (ab248177)
    Western blot - Anti-Themis antibody [EPR7353] - BSA and Azide free (ab248177)
    All lanes : Anti-Themis antibody [EPR7353] (ab126771) at 1/1000 dilution (Purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate
    Lane 3 : Mouse thymus lysate
    Lane 4 : Rat thymus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 73 kDa
    Observed band size: 73 kDa



    This data was developed using ab126771, the same antibody clone in a different buffer formulation.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 25535246 and PMID: 19597499).

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Immunocytochemistry - Anti-Themis antibody [EPR7353] - BSA and Azide free (ab248177)
    Immunocytochemistry - Anti-Themis antibody [EPR7353] - BSA and Azide free (ab248177)

    This data was developed using ab126771, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Themis with Purified ab248177 at 1:50 dilution (10 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-Themis antibody [EPR7353] - BSA and Azide free (ab248177)
    Flow Cytometry - Anti-Themis antibody [EPR7353] - BSA and Azide free (ab248177)

    This data was developed using ab126771, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Themis with Purified ab248177 at 1:50 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Anti-Themis antibody [EPR7353] - BSA and Azide free (ab248177)
    Anti-Themis antibody [EPR7353] - BSA and Azide free (ab248177)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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