Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse Tissue kallikrein ELISA Kit (ab233630)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 4.2 pg/ml
  • Range: 12.5 pg/ml - 800 pg/ml
  • Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse Tissue kallikrein ELISA Kit
    See all Tissue kallikrein kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 7%

  • Sample type

    Serum, Tissue, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    4.2 pg/ml

  • Range

    12.5 pg/ml - 800 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 84 % - %
    Tissue 101 % - %
    Hep Plasma 85 % - %
    EDTA Plasma 94 % - %
    Cit plasma 84 % - %

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse Tissue kallikrein ELISA Kit (ab233630) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tissue kallikrein protein in edta plasma, hep plasma, serum, tissue, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Tissue kallikrein with 4.2 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse Tissue kallikrein ELISA Kit (ab233630)
    Other - Mouse Tissue kallikrein ELISA Kit (ab233630)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of mouse KLK1 standard curve in 1X Cell Extraction Buffer PTR
    Example of mouse KLK1 standard curve in 1X Cell Extraction Buffer PTR

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native KLK1 in mouse serum and plasma samples
    Interpolated concentrations of native KLK1 in mouse serum and plasma samples

    The concentrations of KLK1 were measured in duplicates, interpolated from the KLK1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%, plasma (heparin) 0.03125%, plasma (EDTA) 0.5%, and plasma (citrate) 0.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean KLK1 concentration was determined to be 5,392 pg/mL in serum, 4,932 pg/mL in plasma (heparin), 4,226 pg/mL in plasma (EDTA), and 4,138 pg/mL in plasma (citrate).

  • Interpolated concentrations of native KLK1 in mouse pancreas supernatant based on a 0.03125% extract load
    Interpolated concentrations of native KLK1 in mouse pancreas supernatant based on a 0.03125% extract load

    The concentrations of KLK1 were measured in duplicate and interpolated from the KLK1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean KLK1 concentration was determined to be 129,659 pg/mL in mouse pancreas supernatant sample.

  • Sandwich ELISA - Mouse Tissue kallikrein ELISA Kit (ab233630)
    Sandwich ELISA - Mouse Tissue kallikrein ELISA Kit (ab233630)
    To learn more about the advantages of recombinant antibodies see here.

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