Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse PON2 ELISA Kit (ab208984)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 41 pg/ml
  • Range: 78.13 pg/ml - 5000 pg/ml
  • Sample type: Cell culture extracts, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse PON2 ELISA Kit
    See all PON2 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Mouse spleen 5 3.2%
    Inter-assay
    Sample n Mean SD CV%
    Mouse spleen 3 4.1%

  • Sample type

    Cell culture extracts, Tissue Extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    41 pg/ml

  • Range

    78.13 pg/ml - 5000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 95 93% - 99%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse PON2 ELISA Kit (ab208984) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PON2 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PON2 with 41 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Serum Paraoxonase (PON2) is a 39-45 kDa, calcium binding, membrane-bound protein, ubiquitously expressed in mouse tissue. PON2 exhibits hydrolytic activity against acylhomoserine lactones, which are important bacterial quorum-sensing mediators, which might suggest a role in the defense response to pathogenic bacteria. Mutations of this gene have been suggested to be associated to vascular disease and with a number of quantitative phenotypes related to diabetes.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse PON2 ELISA Kit (ab208984)
    Other - Mouse PON2 ELISA Kit (ab208984)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of mouse PON2 standard curve in 1X Cell Extraction Buffer PTR.
    Example of mouse PON2 standard curve in 1X Cell Extraction Buffer PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native mouse PON2 in mouse liver, spleen, and NIH/3T3 cell extract samples.
    Interpolated concentrations of native mouse PON2 in mouse liver, spleen, and NIH/3T3 cell extract samples.

    Undiluted liver, spleen, and NIH/3T3 extract is at 31,62.5, and 25 µg/mL extract, respectively The concentrations of mouse PON2 were measured in duplicate and interpolated from the mouse PON2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Rat liver extract was diluted 2-fold in 1x Cell Extraction Buffer PTR and assayed for cross-reactivity.
    Rat liver extract was diluted 2-fold in 1x Cell Extraction Buffer PTR and assayed for cross-reactivity.

    Interpolated values from the mouse PON2 protein standard are graphed. Undiluted Rat liver extract is at 12.5 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - Mouse PON2 ELISA Kit (ab208984)
    Sandwich ELISA - Mouse PON2 ELISA Kit (ab208984)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Mouse PON2 ELISA Kit (ab208984)

© 2021 Astana Biomed Group LLP. All rights reserved.