Anti-Translin/TSN antibody (ab96155)
Key features and details
- Rabbit polyclonal to Translin/TSN
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Translin/TSN antibody
See all Translin/TSN primary antibodies -
Description
Rabbit polyclonal to Translin/TSN -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Zebrafish, Orangutan
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Immunogen
Synthetic peptide corresponding to Human Translin/TSN aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab107811) -
Positive control
- This antibody gave a positive signal in the following human tissue lysates: Brain; Testis; Lymph Node as well as the following human whole cell lysates: Jurkat; HeLa; HepG2; MCF7.
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General notes
This product was previously labelled as Translin
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lysates
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab96155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 1 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). Target
- Information by UniProt
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Database links
- Entrez Gene: 7247 Human
- Entrez Gene: 22099 Mouse
- Entrez Gene: 60381 Rat
- Omim: 600575 Human
- SwissProt: P79769 Chicken
- SwissProt: Q08DM8 Cow
- SwissProt: Q15631 Human
- SwissProt: Q62348 Mouse
see all -
Form
Cellular Localisation: Cytoplasm. Nucleus -
Alternative names
- BCLF 1 antibody
- BCLF1 antibody
- Component 3 of promoter of RISC antibody
see all
Images
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Western blot - Anti-Translin/TSN antibody (ab96155)All lanes : Anti-Translin/TSN antibody (ab96155) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TSN knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDaLanes 1-3: Merged signal (red and green). Green - ab96155 observed at 26 kDa. Red - loading control ab8245 observed at 36 kDa.
ab96155 Anti-Translin/TSN antibody was shown to specifically react with Translin/TSN in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264778 (knockout cell lysate ab258255) was used. Wild-type and Translin/TSN knockout samples were subjected to SDS-PAGE. ab96155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Translin/TSN antibody (ab96155)All lanes : Anti-Translin/TSN antibody (ab96155) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : Human brain tissue lysate - total protein (ab29466)
Lane 6 : Human testis tissue lysate - total protein (ab30257)
Lane 7 : Human lymph node tissue lysate - total protein (ab29871)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
Immunocytochemistry/ Immunofluorescence - Anti-Translin/TSN antibody (ab96155)ICC/IF image of ab96155 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96155 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (0)
ab96155 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Translin/TSN antibody (ab96155)All lanes : Anti-Translin/TSN antibody (ab96155) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TSN knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDaLanes 1-3: Merged signal (red and green). Green - ab96155 observed at 26 kDa. Red - loading control ab8245 observed at 36 kDa.
ab96155 Anti-Translin/TSN antibody was shown to specifically react with Translin/TSN in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264778 (knockout cell lysate ab258255) was used. Wild-type and Translin/TSN knockout samples were subjected to SDS-PAGE. ab96155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Western blot - Anti-Translin/TSN antibody (ab96155)All lanes : Anti-Translin/TSN antibody (ab96155) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : Human brain tissue lysate - total protein (ab29466)
Lane 6 : Human testis tissue lysate - total protein (ab30257)
Lane 7 : Human lymph node tissue lysate - total protein (ab29871)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
-
Immunocytochemistry/ Immunofluorescence - Anti-Translin/TSN antibody (ab96155)
ICC/IF image of ab96155 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96155 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
