Mouse PDGF AB ELISA Kit (ab267589)
Key features and details
- Sensitivity: 26 pg/ml
- Range: 24.58 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse PDGF AB ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
26 pg/ml -
Range
24.58 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 133.5 128% - 141% Plasma 139.3 133% - 148% Cell culture media 127.9 119% - 134% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse PDGF AB ELISA Kit is designed for the quantitative determination of PDGF AB in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for mouse PDGF AB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF AB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse PDGF AB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF AB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit detects mouse and human PDGF AB.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 450X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Mouse PDGF AB coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Mouse PDGF AB Detection Antibody 2 vials Mouse PDGF AB Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Relevance
Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains joined by disulphide bonds, termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AB is a 25.5 kDa heterodimer protein consisting of a 13.3 kDa A chain and a 12.2 kDa B chain. -
Cellular localization
Secreted -
Alternative names
- becaplermin
- c-sis
- GDGF
see all -
Database links
- Entrez Gene: 18590 Mouse
- Entrez Gene: 18591 Mouse
- SwissProt: P20033 Mouse
- SwissProt: P31240 Mouse
- Unigene: 2675 Mouse
- Unigene: 144089 Mouse
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.