Mouse NOV ELISA Kit (CCN3) (ab205570)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 51 pg/ml
- Range: 130 pg/ml - 8000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Plasma, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse NOV ELISA Kit (CCN3)
See all CCN3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 8.4% Inter-assay Sample n Mean SD CV% Overall 3 5.9% -
Sample type
Cell culture supernatant, Plasma, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
51 pg/ml -
Range
130 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 111 107% - 116% Hep Plasma 85 85% - 85% EDTA Plasma 88 86% - 88% Cit plasma 104 103% - 106% Mouse brain extract 113 108% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse NOV ELISA Kit (CCN3) (ab205570) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NOV (CCN3) protein in cell culture supernatant, tissue extracts, plasma, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse NOV (CCN3) with 51 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
NOV is a member of the CCN family of extracellular matrix associated signaling proteins. As a secreted protein, NOV regulates cell survival, adhesion, proliferation, differentiation and migration. In humans, NOV expression is associated with metastasis and poor prognosis.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse NOV Capture Antibody 1 x 600µl 10X Mouse NOV Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse NOV Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 75BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Immediate-early protein likely to play a role in cell growth regulation. -
Tissue specificity
Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type. -
Sequence similarities
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CCN family member 3
- CCN3
- IBP-9
see all -
Database links
- Entrez Gene: 18133 Mouse
- SwissProt: Q64299 Mouse
- Unigene: 5167 Mouse
Images
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Other - Mouse NOV ELISA Kit (CCN3) (ab205570)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse NOV standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse NOV standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse NOV standard curve in Sample Diluent 75BS.Background-subtracted data values (mean +/- SD) are graphed.
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Linearity of dilution of native mouse NOV protein in mouse tissue extracts.The concentrations of mouse NOV were interpolated from the mouse NOV standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted. Interpolated values of NOV are plotted based on an extract load of 250 µg/mL.
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Mouse NOV expression is shown for plasma samples.Native NOV was measured in duplicate in 50% EDTA, heparin and citrate plasma diluted in Sample Diluent 75BS and concentrations interpolated from a standard curve diluted in Sample Diluent 75BS. The interpolated dilution factor corrected values are graphed (mean +/- SD).
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Sandwich ELISA - Mouse NOV ELISA Kit (CCN3) (ab205570)To learn more about the advantages of recombinant antibodies see here.