Anti-HEY1 antibody (ab235173)
Key features and details
- Rabbit polyclonal to HEY1
- Suitable for: WB, IHC
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HEY1 antibody
See all HEY1 primary antibodies -
Description
Rabbit polyclonal to HEY1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC HumanWB MouseHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human cerebellum tissue. Hey1 variant 2 human recombinant protein. WB: A172 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry - Anti-HEY1 antibody (ab235173)
Immunohistochemical analysis of human cerebellum tissue labeling Hey1 with ab235173 at 1/200 dilution (30 minutes at RT), followed by a ready to use anti-Rabbit polyclonal IgG (HRP) (8 minutes at RT). Primary staining is DAB. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval using citrate buffer for 20 minutes.
Panel A: H&E staining.
Panel B: ab235173 at 20X magnification.
Panel C: ab235173 at 40X magnification. -
Western blot - Anti-HEY1 antibody (ab235173)All lanes : Anti-HEY1 antibody (ab235173) at 1/1000 dilution
Lane 1 : Hey1 variant 2 human recombinant protein, 50ng
Lane 2 : A-172 lysate at 35 µg
Lane 3 : Jurkat cell lysate at 35 µg
Lane 4 : Mouse brain tissue lysate at 35 µg
Secondary
All lanes : Goat anti Rabbit IgG [H&L] HRP at 1/70000 dilution
Predicted band size: 33 kDa
Exposure time: 19 secondsImaged on Chemidoc program.
