Anti-HEY1 antibody (ab111723)
Key features and details
- Rabbit polyclonal to HEY1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HEY1 antibody
See all HEY1 primary antibodies -
Description
Rabbit polyclonal to HEY1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in Human Spinal Cord tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatChimpanzeeMacaque monkeyOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.Notes WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.Target
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Function
Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6. -
Tissue specificity
Expressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs. -
Sequence similarities
Belongs to the HEY family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23462 Human
- Entrez Gene: 15213 Mouse
- Entrez Gene: 155437 Rat
- Omim: 602953 Human
- SwissProt: Q9Y5J3 Human
- SwissProt: Q9WV93 Mouse
- Unigene: 234434 Human
- Unigene: 29581 Mouse
see all -
Alternative names
- Basic helix loop helix protein OAF1 antibody
- bHLHb31 antibody
- Cardiovascular helix loop-helix factor 2 antibody
see all
Images
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Western blot - Anti-HEY1 antibody (ab111723)Anti-HEY1 antibody (ab111723) at 1 µg/ml + Spinal Cord (Human) Tissue Lysate - adult normal tissue at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab111723 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (1)
ab111723 has been referenced in 1 publication.
- Li S et al. Tumor-educated B cells promote renal cancer metastasis via inducing the IL-1ß/HIF-2a/Notch1 signals. Cell Death Dis 11:163 (2020). PubMed: 32123166
Images
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Western blot - Anti-HEY1 antibody (ab111723)Anti-HEY1 antibody (ab111723) at 1 µg/ml + Spinal Cord (Human) Tissue Lysate - adult normal tissue at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab111723 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
