Mouse Loading Control Antibody Panel (Alexa Fluor® 680) (ab199716)
Overview
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Product name
Mouse Loading Control Antibody Panel (Alexa Fluor® 680)
See all Loading Control Panel kits -
Product overview
ab199716 is a sampler pack of loading control antibodies conjugated to Alexa Fluor® 680.
This panel contains sample sizes of primary antibodies against the following housekeeping targets: alpha Tubulin, GAPDH, and beta Actin.
The Mouse Loading Control Antibody Panel (Alexa Fluor® 680) is designed for validation and confirmation of western blot analysis when tested in conjunction with your proteins of interest.
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Notes
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols.
Images
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Western blot - Mouse Loading Control Antibody Panel (Alexa Fluor® 680) (ab199716)All lanes : Alexa Fluor® 680 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184093) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Observed band size: 50 kDa why is the actual band size different from the predicted?
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab184093 overnight at 4°C. Antibody binding was detected after washing to remove excess antibody and imaged using the Licor Odyssey CLx. -
Western blot - Mouse Loading Control Antibody Panel (Alexa Fluor® 680) (ab199716)All lanes : Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab184095) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Observed band size: 37 kDa why is the actual band size different from the predicted?
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab184093 overnight at 4°C. Antibody binding was detected after washing to remove excess antibody and imaged using the Licor Odyssey CLx. -
Western blot - Mouse Loading Control Antibody Panel (Alexa Fluor® 680) (ab199716)All lanes : Alexa Fluor® 680 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184092) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Observed band size: 42 kDa why is the actual band size different from the predicted?
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab184092 overnight at 4°C. Antibody binding was detected after washing to remove excess antibody and imaged using the Licor Odyssey CLx. -
Mouse Loading Control Antibody Panel (Alexa Fluor® 680) (ab199716)
