Mouse Loading Control Antibody Panel (ab199713)
Overview
-
Product name
Mouse Loading Control Antibody Panel
See all Loading Control Panel kits -
Product overview
Ab199713 is a sampler pack of loading control mouse antibodies and a Goat Anti-Mouse IgG secondary antibody conjugated to HRP.
This panel contains sample sizes of all mouse host primary antibodies against the following housekeeping targets: alpha Tubulin, GAPDH, COXIV, Histone H3, alpha 1 NaK ATPase.The Mouse Loading Control Antibody Panel is designed for validation and confirmation of western blot analysis when tested in conjunction with your proteins of interest.
Please refer to each individual component datasheet for storage instructions.
-
Notes
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Refer to the 'Associated Products' section below to see HRP-conjugated versions of these antibodies.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols.
Images
-
All lanes : Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)
Observed band size: 50 kDa why is the actual band size different from the predicted?
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406 -
Lanes 1-5 : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) ((Blocked in 5% MILK))
Lanes 6-10 : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
Lanes 1 & 6 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lanes 2 & 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lanes 3 & 8 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lanes 4 & 9 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lanes 5 & 10 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Mouse (HRP conjugated) at 1/5000 dilution
Observed band size: 40 kDa why is the actual band size different from the predicted?
The membrane 1-5 was blocked in 5% Milk (1 hour). The membrane 6-10 was blocked in 5% BSA (1 hour). Abcam routinely uses 5% BSA to block however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent and significantly decreases the signal on the membrane. We have informed all users of the antibody of this finding. -
All lanes : Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)
Lane 4 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 5 : Kidney (Cow) Tissue Lysate (ab29073)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size: 15 kDa why is the actual band size different from the predicted?
-
All lanes : Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : Testis (Mouse) Tissue Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 4 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Observed band size: 18 kDa why is the actual band size different from the predicted?
-
Lanes 1-3 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1 µg/ml
Lanes 4-6 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 2 µg/ml
Lanes 7-9 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 5 µg/ml
Lanes 1 & 7 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lanes 2 & 5 & 8 : Brain (Mouse) Tissue Lysate (ab27253)
Lanes 3 & 6 & 9 : Brain (Rat) Tissue Lysate (ab7942)
Lane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lysates/proteins at 20 µg per lane.
Observed band size: 112 kDa why is the actual band size different from the predicted?
-