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Neuroscience Neurotransmission Nitric Oxide NOS

Mouse iNOS Antibody Pair - BSA and Azide free (ab256722)

Mouse iNOS Antibody Pair - BSA and Azide free (ab256722)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse iNOS Antibody Pair - BSA and Azide free
    See all iNOS kits
  • Assay type

    ELISA set
  • Range

    46.88 pg/ml - 3000 pg/ml
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    The Antibody Pair can be used to quantify Mouse iNOS. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab253219), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Mouse iNOS Capture Antibody (unconjugated) 1 x 100µg
    Mouse iNOS Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Nitric Oxide
    • NOS
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
  • Function

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
  • Tissue specificity

    Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
  • Sequence similarities

    Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Target information above from: UniProt accession P35228 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • HEP-NOS
    • Hepatocyte NOS
    • HEPNOS
    • inducible
    • Inducible nitric oxide synthase
    • Inducible NO synthase
    • Inducible NOS
    • iNOS
    • MAC NOS
    • Macrophage NOS
    • Nitric oxide synthase
    • Nitric oxide synthase 2 inducible
    • Nitric oxide synthase 2 inducible macrophage
    • nitric oxide synthase 2A (inducible, hepatocytes)
    • Nitric oxide synthase inducible
    • nitric oxide synthase, macrophage
    • NOS
    • NOS 2
    • Nos II
    • NOS type II
    • nos2
    • NOS2_HUMAN
    • NOS2A
    • NOS2A, Inducible, Hepatocyte
    • Peptidyl-cysteine S-nitrosylase NOS2
    see all
  • Database links

    • Entrez Gene: 18126 Mouse
    • SwissProt: P29477 Mouse
    • Unigene: 2893 Mouse

    Images

    • Sandwich ELISA - Mouse iNOS Antibody Pair - BSA and Azide free (ab256722)
      Sandwich ELISA - Mouse iNOS Antibody Pair - BSA and Azide free (ab256722)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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