Mouse iNOS Antibody Pair - BSA and Azide free (ab256722)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse
Overview
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Product name
Mouse iNOS Antibody Pair - BSA and Azide free
See all iNOS kits -
Assay type
ELISA set -
Range
46.88 pg/ml - 3000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
The Antibody Pair can be used to quantify Mouse iNOS. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab253219), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Mouse iNOS Capture Antibody (unconjugated) 1 x 100µg Mouse iNOS Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. -
Tissue specificity
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. -
Sequence similarities
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. - Information by UniProt
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Alternative names
- HEP-NOS
- Hepatocyte NOS
- HEPNOS
see all -
Database links
- Entrez Gene: 18126 Mouse
- SwissProt: P29477 Mouse
- Unigene: 2893 Mouse
Images
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To learn more about the advantages of recombinant antibodies see here.