Mouse ICAM 1 ELISA Kit (ab252355)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 28.15 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Culture Media
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse ICAM 1 ELISA Kit
See all ICAM1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.6% Inter-assay Sample n Mean SD CV% Serum 3 4% -
Sample type
Cell culture supernatant, Serum, Tissue Culture Media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
28.15 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 113 107% - 115% Serum 96 81% - 111% Tissue Extracts 91 82% - 99% Hep Plasma 91 84% - 100% EDTA Plasma 99 90% - 104% Cit plasma 102 86% - 113% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse ICAM 1 ELISA Kit (ab252355) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ICAM 1 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse ICAM 1 with 28.15 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
ICAM1, is a cell surface glycoprotein typically expressed in endothelial cells and cells of the immune system. The extracellular portion of ICAM-1 forms five immunoglobulin like domains attached to a single hydrophobic transmembrane region and a short cytoplasmic tail. ICAM-1, binds to the Leukocyte Integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) as well as to non-integrin ligands such as CD43, fibrinogen, hyaluronan, Rhinoviruses and Plasmodium falciparum-infected erythrocytes. Binding to LFA-1 facilitates trans-endothelial leukocyte migration to areas of inflammation via promotion of endothelial apical cups assembly. Mouse ICAM1 shares 92.6 % and 48.6 % sequence homology with rat and human ICAM1, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse ICAM 1 Capture Antibody 1 x 600µl 10X Mouse ICAM 1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Mouse ICAM 1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsMonoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Antigen identified by monoclonal antibody BB2
- BB 2
- BB2
see all -
Database links
- Entrez Gene: 15894 Mouse
- SwissProt: P13597 Mouse
- Unigene: 435508 Mouse
Images
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Other - Mouse ICAM 1 ELISA Kit (ab252355)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse ICAM 1 Standard Curve ComparisonStandard Curve comparison between human Mouse ICAM 1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of mouse ICAM 1 standard curve in Sample Diluent NS.The ICAM 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse ICAM 1 standard curve in 1X Cell Extraction Buffer PTR.The ICAM 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native ICAM 1 in mouse serum, plasma samples.The concentrations of ICAM 1 were measured in duplicates, interpolated from the ICAM 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (citrate) 1:200, plasma (EDTA) 1:200 and plasma (heparin) 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM 1 concentration was determined to be 506.72 ng/mL in serum, 535.54 ng/mL in plasma (citrate), 498.48 ng/mL in plasma (EDTA), 563.77 ng/mL in and plasma (heparin).
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Interpolated concentrations of native ICAM 1 in LPS stimulated J774A.1 cell culture supernatant, unstimulated J774A.1 cell culture supernatant, and spleen cell culture supernatant samples.The concentrations of ICAM 1 were measured in duplicates, interpolated from the ICAM 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: LPS stimulated J774A.1 cell culture supernatant 50%, unstimulated J774A.1 cell culture supernatant 50%, and spleen cell culture supernatant 4%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM 1 concentration was determined to be 4.59 ng/mL in LPS stimulated J774A.1 cell culture supernatant, 3.23 ng/mL in unstimulated J774A.1 cell culture supernatant, and 10.94 ng/mL in and spleen cell culture supernatant.
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Interpolated concentrations of native ICAM 1 in mouse spleen and mouse lung based on a 10 µg/mL extract load.The concentrations of ICAM 1 were measured in duplicate and interpolated from the ICAM 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM 1 concentration was determined to be 1341.51 pg/mL in spleen extract and 3625 pg/mL in lung extract.
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Sandwich ELISA - Mouse ICAM 1 ELISA Kit (ab252355)To learn more about the advantages of recombinant antibodies see here.
