Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse ICAM 1 ELISA Kit (ab252355)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 28.15 pg/ml
  • Range: 62.5 pg/ml - 4000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Culture Media
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse ICAM 1 ELISA Kit
    See all ICAM1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 4.6%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 4%

  • Sample type

    Cell culture supernatant, Serum, Tissue Culture Media, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    28.15 pg/ml

  • Range

    62.5 pg/ml - 4000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 113 107% - 115%
    Serum 96 81% - 111%
    Tissue Extracts 91 82% - 99%
    Hep Plasma 91 84% - 100%
    EDTA Plasma 99 90% - 104%
    Cit plasma 102 86% - 113%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow

  • Product overview

    Mouse ICAM 1 ELISA Kit (ab252355) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ICAM 1 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse ICAM 1 with 28.15 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    ICAM1, is a cell surface glycoprotein typically expressed in endothelial cells and cells of the immune system.  The extracellular portion of ICAM-1 forms five immunoglobulin like domains attached to a single hydrophobic transmembrane region and a short cytoplasmic tail.  ICAM-1, binds to the Leukocyte Integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) as well as to non-integrin ligands such as CD43, fibrinogen, hyaluronan, Rhinoviruses and Plasmodium falciparum-infected erythrocytes.  Binding to LFA-1 facilitates trans-endothelial leukocyte migration to areas of inflammation via promotion of endothelial apical cups assembly. Mouse ICAM1 shares 92.6 % and 48.6 % sequence homology with rat and human ICAM1, respectively.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse ICAM 1 ELISA Kit (ab252355)
    Other - Mouse ICAM 1 ELISA Kit (ab252355)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Mouse ICAM 1 Standard Curve Comparison
    Mouse ICAM 1 Standard Curve Comparison

    Standard Curve comparison between human Mouse ICAM 1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • Example of mouse ICAM 1 standard curve in Sample Diluent NS.
    Example of mouse ICAM 1 standard curve in Sample Diluent NS.

    The ICAM 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Example of mouse ICAM 1 standard curve in 1X Cell Extraction Buffer PTR.
    Example of mouse ICAM 1 standard curve in 1X Cell Extraction Buffer PTR.

    The ICAM 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native ICAM 1 in mouse serum, plasma samples.
    Interpolated concentrations of native ICAM 1 in mouse serum, plasma samples.

    The concentrations of ICAM 1 were measured in duplicates, interpolated from the ICAM 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (citrate) 1:200, plasma (EDTA) 1:200 and plasma (heparin) 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM 1 concentration was determined to be 506.72 ng/mL in serum, 535.54 ng/mL in plasma (citrate), 498.48 ng/mL in plasma (EDTA), 563.77 ng/mL in and plasma (heparin).

  • Interpolated concentrations of native ICAM 1 in LPS stimulated J774A.1 cell culture supernatant, unstimulated J774A.1 cell culture supernatant, and spleen cell culture supernatant samples.
    Interpolated concentrations of native ICAM 1 in LPS stimulated J774A.1 cell culture supernatant, unstimulated J774A.1 cell culture supernatant, and spleen cell culture supernatant samples.

    The concentrations of ICAM 1 were measured in duplicates, interpolated from the ICAM 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: LPS stimulated J774A.1 cell culture supernatant 50%, unstimulated J774A.1 cell culture supernatant 50%, and spleen cell culture supernatant 4%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM 1 concentration was determined to be 4.59 ng/mL in LPS stimulated J774A.1 cell culture supernatant, 3.23 ng/mL in unstimulated J774A.1 cell culture supernatant, and 10.94 ng/mL in and spleen cell culture supernatant.

  • Interpolated concentrations of native ICAM 1 in mouse spleen and mouse lung based on a 10 µg/mL extract load.
    Interpolated concentrations of native ICAM 1 in mouse spleen and mouse lung based on a 10 µg/mL extract load.

    The concentrations of ICAM 1 were measured in duplicate and interpolated from the ICAM 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM 1 concentration was determined to be 1341.51 pg/mL in spleen extract and 3625 pg/mL in lung extract.

  • Sandwich ELISA - Mouse ICAM 1 ELISA Kit (ab252355)
    Sandwich ELISA - Mouse ICAM 1 ELISA Kit (ab252355)
    To learn more about the advantages of recombinant antibodies see here.

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