Anti-R2D2 antibody (ab27650)
Key features and details
- Rabbit polyclonal to R2D2
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
Overview
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Product name
Anti-R2D2 antibody -
Description
Rabbit polyclonal to R2D2 -
Host species
Rabbit -
Specificity
The antibody specifically recognises the immunising peptide in a blocking western blot. All four observed bands are blocked, showing that they are specific. There is also low background and high titre in ELISA. Please let us know if you can explain these bands. -
Tested Applications & Species
See all applications and species dataApplication Species WB Drosophila melanogaster
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Immunogen
Synthetic peptide corresponding to Drosophila melanogaster R2D2 aa 100-200 (intracellular) conjugated to keyhole limpet haemocyanin.
(Peptide available asab17670) -
Positive control
- This antibody gave a positive signal in Drosophila lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB Drosophila melanogasterApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).Target
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Relevance
The RNA interference (RNAi) pathway is initiated by processing long double-stranded RNA into small interfering RNA (siRNA). The siRNA-generating enzyme consists of DCR-2 and R2D2. R2D2 is homologous to the Caenorhabditis elegans RNAi protein RDE-4. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 34066 Drosophila melanogaster
- SwissProt: Q9VLW8 Drosophila melanogaster
- Unigene: 10740 Drosophila melanogaster
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Alternative names
- CG7138 antibody
- CG7138 PA antibody
- LD06392p antibody
Images
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Western blot - Anti-R2D2 antibody (ab27650)All lanes : Anti-R2D2 antibody (ab27650) at 1 µg/ml
Lane 1 : Drosophila lysate
Lane 2 : Drosophila lysate with Human R2D2 peptide (ab17670) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size: 35 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 39 kDa (possible cross reactivity), 55 kDa (possible cross reactivity), 85 kDa (possible cross reactivity)
ab27650 specifically recognises a band at 37kDa corresponding to R2D2, which is blocked using the immunising peptide (ab17670).
Datasheets and documents
References (0)
ab27650 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-R2D2 antibody (ab27650)All lanes : Anti-R2D2 antibody (ab27650) at 1 µg/ml
Lane 1 : Drosophila lysate
Lane 2 : Drosophila lysate with Human R2D2 peptide (ab17670) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size: 35 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 39 kDa (possible cross reactivity), 55 kDa (possible cross reactivity), 85 kDa (possible cross reactivity)
ab27650 specifically recognises a band at 37kDa corresponding to R2D2, which is blocked using the immunising peptide (ab17670).