Human Growth Hormone ELISA Kit (ab190811)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.6 pg/ml
- Range: 9.4 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Growth Hormone ELISA Kit
See all Growth Hormone kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 3.6% Inter-assay Sample n Mean SD CV% serum 3 2.4% -
Sample type
Cell culture supernatant, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.6 pg/ml -
Range
9.4 pg/ml - 600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Milk 94 91% - 100% Urine 112 107% - 119% Serum 103 97% - 109% Cell culture media 93 90% - 98% Hep Plasma 97 94% - 99% EDTA Plasma 93 87% - 98% Cit plasma 97 92% - 102% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human Growth Hormone ELISA Kit (ab190811) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Growth Hormone protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Growth Hormone with 1.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY
This kit recognizes both native and recombinant human Growth Hormone protein in serum, plasma, milk, urine, and cell culture supernatant samples only.
Saliva, and cell and tissue extract samples have not been tested with this kit.
SPECIES REACTIVITY
This kit recognizes human Growth Hormone protein.
Other species reactivity was determined by measuring neat serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.
Reactivity
- Mouse
- Rat
- Cow
CALIBRATION
This immunoassay is calibrated against a highly purified human Growth Hormone. The NIBSC/WHO unclassified purified human Growth Hormone preparation 80/505 was evaluated in this kit.
The dose response curve of the unclassified standard Growth Hormone parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep Human Growth Hormone kit to approximate NIBSC 80/505 units, use the equation below.
NIBSC (80/505) approximate value (mIU/mL) = 2.3x10-6 x SimpleStep Human Growth Hormone value (pg/mL).
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Notes
Growth Hormone (also known as GH, GH1, Somatotropin and Pituitary growth hormone) is a circulations hormone that plays an important role in somatic growth control. Growth Hormone binds to the Growth Hormone Receptor present in a variety of tissues and induces signaling cascades. Growth Hormone secretion is controlled positively and negatively by other hormones, including Ghrelin and Somatostatin. Overproduction of Growth Hormone can result in gigantism whereas deficiency can contribute to dwarfism.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Growth Hormone Capture Antibody 1 x 600µl 10X Human Growth Hormone Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Growth Hormone Lyophilized Recombinant Protein (ab116162) 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. -
Involvement in disease
Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy. -
Sequence similarities
Belongs to the somatotropin/prolactin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- gH
- GH-N
- GH1
see all -
Database links
- Entrez Gene: 2688 Human
- Omim: 139250 Human
- SwissProt: P01241 Human
- Unigene: 655229 Human
Images
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Other - Human Growth Hormone ELISA Kit (ab190811)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Growth Hormone Standard Curve ComparisonStandard Curve comparison between human Growth Hormone SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Human Growth Hormone Standard Curve ComparisonStandard Curve comparison between human Growth Hormone new SimpleStep ELISA kit and previous ELISA kit. The new SimpleStep ELISA kit shows increased sensitivity.
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. Example of human Growth Hormone standard curve in Sample Diluent NS.The Growth Hormone standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Standard curveExample of human Growth Hormone standard curve in Sample Diluent NS. The Growth Hormone standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Growth Hormone in human normal mixed gender serum and pregnant serum samples.The concentrations of Growth Hormone were measured in duplicates, interpolated from the Growth Hormone standard curves and corrected for sample dilution. Undiluted samples are as follows: mixed gender serum 100% and pregnant serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Growth Hormone concentration was determined to be 203.3 pg/mL in neat pooled mixed gender serum and 5,327 pg/mL in neat pooled pregnant female serum.
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Interpolated concentrations of native Growth Hormone in human plasma samples.The concentrations of Growth Hormone were measured in duplicates, interpolated from the Growth Hormone standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Growth Hormone concentration was determined to be 309.0 pg/mL in neat plasma (citrate), 329.1 pg/mL in neat plasma (heparin), and 120 pg/mL in and neat plasma (EDTA).
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Serum from nine individual healthy human male and female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Linearity of dilution.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Growth Hormone was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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Linearity of dilution.Recombinant Growth Hormone was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.
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Sandwich ELISA - Human Growth Hormone ELISA Kit (ab190811)To learn more about the advantages of recombinant antibodies see here.
