Mouse GREM2 ELISA kit (PDRC) (ab267575)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 3.28 pg/ml - 800 pg/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse GREM2 ELISA kit (PDRC) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
3 pg/ml -
Range
3.28 pg/ml - 800 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 107 85% - 146% Plasma 77 68% - 113% Cell culture media 94 89% - 102% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse GREM2 ELISA Kit is designed for the quantitative determination of GREM2 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for mouse GREM2 coated on a 96-well plate. Standards and samples are pipetted into the wells and GREM2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse GREM2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GREM2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Mouse GREM2 coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Mouse GREM2 Detection Antibody 2 vials Mouse GREM2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Cytokine that inhibits the activity of BMP2 and BMP4 in a dose-dependent manner. Antagonized BMP4-induced suppression of progesterone production in granulosa cells. -
Sequence similarities
Belongs to the DAN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- BMP antagonist 2
- CKTSF1B2
- Cysteine knot superfamily 1
see all -
Database links
- Entrez Gene: 23893 Mouse
- SwissProt: O88273 Mouse
- Unigene: 25760 Mouse
Images
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Example standard curve
These standard curves are for demonstration only. A standard curve must be run with each assay.