Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human IGFBP4 ELISA Kit (ab230936)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 12 pg/ml
  • Range: 39.1 pg/well - 2500 pg/well
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human IGFBP4 ELISA Kit
    See all IGFBP4 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Plasma 5 4.8%
    Inter-assay
    Sample n Mean SD CV%
    Plasma 3 6.1%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    12 pg/ml

  • Range

    39.1 pg/well - 2500 pg/well

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 107 105% - 110%
    Serum 114 106% - 125%
    Hep Plasma 101 99% - 101%
    EDTA Plasma 112 101% - 127%
    Cit plasma 108 101% - 120%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human IGFBP4 ELISA Kit (ab230936) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGFBP4 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IGFBP4 with 12 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Human IGFBP4 ELISA Kit (ab230936)
    Other - Human IGFBP4 ELISA Kit (ab230936)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human IGFBP4 standard curve in Sample Diluent NS
    Example of human IGFBP4 standard curve in Sample Diluent NS

    The IGFBP4 standard curve was prepared as described in Section 10.

  • Interpolated concentrations of native IGFBP4 in human serum, plasma and cell culture supernatant samples.
    Interpolated concentrations of native IGFBP4 in human serum, plasma and cell culture supernatant samples.

    The concentrations of IGFBP4 were measured in duplicates, interpolated from the IGFBP4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (citrate) 1:200, plasma (heparin) 1:200, plasma (EDTA) 1:200, and A549 supernatant 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP4 concentration was determined to be 452 ng/mL in serum, 336 ng/mL in plasma (citrate), 236 ng/ml in plasma (heparin), 359 ng/ml in plasma (EDTA), and 410 ng/mL in A549 supernatant.

  • Serum from ten individual healthy human male donors was measured in duplicate.
    Serum from ten individual healthy human male donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP4 concentration was determined to be 478 ng/mL with a range of 389 – 743 ng/mL.

  • Sandwich ELISA - Human IGFBP4 ELISA Kit (ab230936)
    Sandwich ELISA - Human IGFBP4 ELISA Kit (ab230936)
    To learn more about the advantages of recombinant antibodies see here.

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