Human Bone Sialoprotein Antibody Pair - BSA and Azide free (IBSP) (ab253479)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human Bone Sialoprotein Antibody Pair - BSA and Azide free (IBSP)
See all Bone Sialoprotein kits -
Assay type
ELISA set -
Range
21.875 pg/ml - 1400 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Both capture and detector antibodies are recombinant rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
The Antibody Pair can be used to quantify Human Bone Sialoprotein. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab277706), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human Bone Sialoprotein (IBSP) Capture Antibody (unconjugated) 1 x 100µg Human Bone Sialoprotein (IBSP) Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment. -
Post-translational
modificationsN-glycosylated; glycans consist of sialated and core-fucosylated bi-, tri- and tetraantennary chains.
O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.
Sulfated on either Tyr-313 or Tyr-314. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- BNSP
- Bone sialoprotein 2
- Bone sialoprotein II
see all -
Database links
- Entrez Gene: 3381 Human
- Omim: 147563 Human
- SwissProt: P21815 Human
- Unigene: 518726 Human
Images
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Example of Human Bone Sialoprotein (IBSP) standard curve.
Representative standard curve from corresponding SimpleStep ELISA® Kit (ab277706), which uses the same antibody pair. Note that the reference range provided for the pair is an estimation based on the performance of the related kit.
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Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253479)To learn more about the advantages of recombinant antibodies see here.
