Mouse CSF-1-R ELISA Kit (ab240681)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.04 ng/ml
- Range: 0.16 ng/ml - 10 ng/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CSF-1-R ELISA Kit
See all CSF-1-R kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.8% Inter-assay Sample n Mean SD CV% Serum 3 4% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.04 ng/ml -
Range
0.16 ng/ml - 10 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 92 85% - 97% Serum 108 102% - 111% Cell culture extracts 118 116% - 119% Tissue Extracts 118 111% - 132% Hep Plasma 105 101% - 108% EDTA Plasma 102 95% - 109% Cit plasma 108 106% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse CSF-1-R ELISA Kit (ab240681) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CSF-1-R protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CSF-1-R with 0.04 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CSF-1-R Capture Antibody 1 x 600µl 10X Mouse CSF-1-R Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Mouse CSF-1-R Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34. -
Tissue specificity
Expressed in bone marrow and in differentiated blood mononuclear cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- C FMS
- CD 115
- CD115
see all -
Database links
- Entrez Gene: 12978 Mouse
- SwissProt: P09581 Mouse
- Unigene: 22574 Mouse
Images
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Other - Mouse CSF-1-R ELISA Kit (ab240681)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse CSF-1-R standard curve in Sample Diluent NS.The CSF-1-R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse CSF-1-R standard curve in 1X Cell Extraction Buffer PTR.The CSF-1-R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CSF-1-R in mouse serum, plasma (EDTA), plasma (heparin), and plasma (citrate) samples.The concentrations of CSF-1-R were measured in duplicates, interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:3,200, plasma (EDTA) 1:3,200, plasma (heparin) 1:3,200, and plasma (citrate) 1:1,600. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration was determined to be 6.8 µg/mL in serum, 6.9 µg/mL in plasma (EDTA), 6.8 µg/mL in plasma (heparin), and 3.9 µg/mL in plasma (citrate).
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Interpolated concentrations of native CSF-1-R in mouse spleen cell supernatant, liver cell supernatant, and RAW cell supernatant samples.The concentrations of CSF-1-R were measured in duplicates, interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows: spleen cell supernatant 2.5%, liver cell supernatant 25%, and RAW cell supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration was determined to be 78.9 ng/mL in neat spleen cell supernatant, 13 ng/mL in neat liver cell supernatant, and 1,024 ng/mL in neat RAW cell supernatant.
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Interpolated concentrations of native CSF-1-R in mouse spleen cell extract and RAW 264.7 cell extract samples.The concentrations of CSF-1-R were measured in duplicates, interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows: spleen cell extract 75 µg/mL and RAW264.7 cell extract 37.5 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Mouse CSF-1-R ELISA Kit (ab240681)To learn more about the advantages of recombinant antibodies see here.
