Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human DBI knockout HEK-293T cell lysate (ab258389)

Overview

  • Product name

    Human DBI knockout HEK-293T cell lysate

  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 3.

  • Passage number

  • Knockout validation

    Sanger Sequencing

  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Properties

Target

  • Function

    Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.

  • Tissue specificity

    Isoform 1 is ubiquitous, with a moderate expression level. Isoform 2 is ubiquitous with high level in liver and adipose tissue. Isoform 3 is ubiquitous with strong expression in adipose tissue and heart.

  • Sequence similarities

    Belongs to the ACBP family.
    Contains 1 ACB (acyl-CoA-binding) domain.
  • Information by UniProt

  • Alternative names

    • ACBD 1
    • ACBD1
    • ACBP
    • ACBP_HUMAN
    • Acyl CoA binding domain containing 1
    • Acyl CoA binding protein
    • Acyl Coenzyme A binding domain containing 1
    • Acyl coenzyme A binding protein
    • Acyl-CoA-binding protein
    • CCK RP
    • CCKRP
    • Cholecystokinin releasing peptide trypsin sensitive
    • DBI
    • Diazepam-binding inhibitor
    • Endozepine
    • EP
    • GABA receptor modulator
    • MGC70414
    see all

Properties

Images

  • Sanger Sequencing - Human DBI knockout HEK293T cell lysate (ab258389)
    Sanger Sequencing - Human DBI knockout HEK293T cell lysate (ab258389)
    Homozygous: 14 bp deletion in exon 3

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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