Mouse CD23 Matched Antibody Pair Kit (ab216065)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 15.61 pg/ml - 1000 pg/ml
Overview
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Product name
Mouse CD23 Matched Antibody Pair Kit
See all CD23 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
7.8 pg/ml -
Range
15.61 pg/ml - 1000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse CD23 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse CD23.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab206984. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse CD23 Capture Antibody 2 x 50µg 1 x 50µg Mouse CD23 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse CD23 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane. Secreted. Also exists as a soluble excreted form. - Information by UniProt
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Alternative names
- Blast 2
- BLAST-2
- Blast2
see all -
Database links
- Entrez Gene: 14128 Mouse
- SwissProt: P20693 Mouse
Images
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Mouse CD23 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse CD23 Matched Antibody Pair Kit (ab216065)To learn more about the advantages of recombinant antibodies see here.