Mouse CCL28 ELISA Kit (ab210578)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.7 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CCL28 ELISA Kit
See all CCL28/MEC kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Saliva 8 2.6% Inter-assay Sample n Mean SD CV% Saliva 3 6.9% -
Sample type
Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
4.7 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 116 115% - 118% Serum 111 99% - 118% Hep Plasma 99 96% - 100% EDTA Plasma 109 105% - 112% Cit plasma 99 94% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Rat, Human -
Product overview
Mouse CCL28 ELISA Kit (ab210578) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL28 protein in cell culture extracts, cell culture supernatant, plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CCL28 with 4.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Mouse CCL28 is a member of the CC subfamily of chemokines. Mucosal epithelial cells such as exocrine glands, trachea, and colon express mouse CCL28 protein. In lower levels, mouse CCL28 is also found in brain, spleen and lung tissues. CCL28 chemoattracts resting CD4 and CD8 cells, as well as IgA antibody secreting cells. Furthermore, CCL28 plays a role in extravasation of IgA antibody secreting cells in the small intestine, colon, and lactating mammary gland. Human and mouse CCL28 share an overall 66% amino acid sequence identity, with an 83% amino acid sequence identity in their mature regions.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CCL28 Capture Antibody 1 x 600µl 10X Mouse CCL28 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Mouse CCL28 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NBP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. -
Tissue specificity
Preferentially expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. Also found in prostate, spleen, thyroid, psoriasis skin and in lower levels in peripheral blood leukocytes, small intestine, Peyer patches, stomach and normal skin. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C C motif chemokine ligand 28
- C-C motif chemokine 28
- CC chemokine CCL28
see all -
Database links
- Entrez Gene: 56838 Mouse
- SwissProt: Q9JIL2 Mouse
- Unigene: 143745 Mouse
Images
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Other - Mouse CCL28 ELISA Kit (ab210578)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse CCL28 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse CCL28 standard curve in Sample Diluent 20BP.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse CCL28 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked CCL28 in mouse serum and plasma samples.The concentrations of CCL28 were measured in duplicates, interpolated from the CCL28 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL28 concentration was determined to be 4,718.7 pg/mL in serum, 4,395.5 pg/mL in plasma (citrate), 4,697.8 pg/mL in plasma (EDTA) and 5,130.8 pg/mL in plasma (heparin).
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Interpolated concentrations of native CCL28 in mouse serum and plasma samples.The concentrations of CCL28 were measured in duplicates, interpolated from the CCL28 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL28 concentration was determined to be 461.6 pg/mL in serum, 615.2 pg/mL in plasma (citrate), 609.5 pg/mL in plasma (EDTA), and 390.0 pg/mL in plasma (heparin).
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Sandwich ELISA - Mouse CCL28 ELISA Kit (ab210578)To learn more about the advantages of recombinant antibodies see here.
