Mouse CCL28 Matched Antibody Pair Kit (ab216061)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
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Product name
Mouse CCL28 Matched Antibody Pair Kit
See all CCL28/MEC kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
37.54 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse CCL28 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse CCL28.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab210578. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse CCL28 Capture Antibody 2 x 50µg 1 x 50µg Mouse CCL28 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse CCL28 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. -
Tissue specificity
Preferentially expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. Also found in prostate, spleen, thyroid, psoriasis skin and in lower levels in peripheral blood leukocytes, small intestine, Peyer patches, stomach and normal skin. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C C motif chemokine ligand 28
- C-C motif chemokine 28
- CC chemokine CCL28
see all -
Database links
- Entrez Gene: 56838 Mouse
- SwissProt: Q9JIL2 Mouse
- Unigene: 143745 Mouse
Images
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Mouse CCL28 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse CCL28 Matched Antibody Pair Kit (ab216061)To learn more about the advantages of recombinant antibodies see here.
