Mouse Beta nerve growth factor ELISA Kit (ab277398)
Key features and details
- Sensitivity: 40 pg/ml
- Range: 0.041 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Beta nerve growth factor ELISA Kit
See all Beta nerve growth factor kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% General Inter-assay Sample n Mean SD CV% General -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
40 pg/ml -
Range
0.041 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 117.8 81% - 149% Plasma 108.8 78% - 148% Cell culture media 108.4 79% - 139% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse
Predicted to work with: Human
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Product overview
Mouse Beta nerve growth factor ELISA Kit (ab277398) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Beta nerve growth factor in serum (mouse beta-NGF concentration is low in normal serum and plasma samples, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse Beta nerve growth factor coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse Beta nerve growth factor present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse Beta nerve growth factor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse Beta nerve growth factor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated Anti-Mouse Beta nerve growth factor Antibody 2 vials Anti-Mouse Beta nerve growth factor coated Microplate 1 unit Mouse Beta nerve growth factor standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons; neurotrophic factor; cutaneous innervation; growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta. -
Cellular localization
Secreted. -
Alternative names
- Beta nerve growth factor
- Beta NGF
- HSAN5
see all -
Database links
- Entrez Gene: 4803 Human
- Entrez Gene: 18049 Mouse
- Omim: 162030 Human
- SwissProt: P01138 Human
- SwissProt: P01139 Mouse
- Unigene: 2561 Human
- Unigene: 1259 Mouse
Images
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Mouse Beta nerve growth factor ELISA kit (ab277398) Standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.