Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CDNF antibody [EPR22334-99] (ab253238)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22334-99] to CDNF
Suitable for: IP, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-CDNF antibody [EPR22334-99]
See all CDNF primary antibodies
Description

Rabbit monoclonal [EPR22334-99] to CDNF
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Recombinant Human CDNF Protein (aa25-187). Rat and human skeletal muscle tissue lysate. Human testis, liver, brain and kidney tissue lysate. IP: Human skeletal muscle tissue and testis lysate..
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22334-99
Isotype

IgG
Research areas

Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] (ab253238)

CDNF was immunoprecipitated from 0.35 mg human testis tissue lysate with ab253238 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab253238 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/1000 dilution.

Lane 1: Human testis tissue lysate 10 µg (input)
Lane 2: ab253238 IP in human testis tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253238 in human testis tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] (ab253238)

CDNF was immunoprecipitated from 0.35 mg human skeletal muscle tissue lysate with ab253238 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab253238 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/1000 dilution.

Lane 1: Human skeletal muscle tissue lysate 10 µg (input)
Lane 2: ab253238 IP in human skeletal muscle tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253238 in human skeletal muscle lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Western blot - Anti-CDNF antibody [EPR22334-99] (ab253238)

All lanes : Anti-CDNF antibody [EPR22334-99] (ab253238) at 1/1000 dilution

Lane 1 : Human liver tissue lysate
Lane 2 : Human brain tissue lysate
Lane 3 : Human kidney tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 21 kDa

Exposure time: 3 minutes

Blocking/diluting buffer and concentration: 5% NFDM/TBST.

Kidney and brain are CDNF low expressing tissues (PMID:17611540).

This blot was developed using a higher sensitivity ECL substrate.
Western blot - Anti-CDNF antibody [EPR22334-99] (ab253238)

All lanes : Anti-CDNF antibody [EPR22334-99] (ab253238) at 1/1000 dilution

Lane 1 : Mouse skeletal muscle tissue lysate
Lane 2 : Rat skeletal muscle lysate
Lane 3 : Human skeletal muscle lysate
Lane 4 : Human testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 3-4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 21 kDa
Observed band size: 17 kDa
why is the actual band size different from the predicted?

Exposure times: Lanes 1 and 2: 37 seconds; Lanes 3 and 4: 3 minutes.

Blocking/diluting buffer and concentration: 5% NFDM/TBST

The expression profile and molecular weight observed are consistent with literature (PMID: 17611540).
Western blot - Anti-CDNF antibody [EPR22334-99] (ab253238)

Anti-CDNF antibody [EPR22334-99] (ab253238) at 1/1000 dilution + Recombinant Human CDNF Protein (aa25-187) 10 ng

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 21 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Anti-CDNF antibody [EPR22334-99] (ab253238)

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