Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDNF antibody [EPR22334-99] (ab253238)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22334-99] to CDNF
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CDNF antibody [EPR22334-99]
    See all CDNF primary antibodies

  • Description

    Rabbit monoclonal [EPR22334-99] to CDNF

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Recombinant Human CDNF Protein (aa25-187). Rat and human skeletal muscle tissue lysate. Human testis, liver, brain and kidney tissue lysate. IP: Human skeletal muscle tissue and testis lysate..

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] (ab253238)
    Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] (ab253238)

    CDNF was immunoprecipitated from 0.35 mg human testis tissue lysate with ab253238 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab253238 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/1000 dilution.

    Lane 1: Human testis tissue lysate 10 µg (input)
    Lane 2: ab253238 IP in human testis tissue lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253238 in human testis tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] (ab253238)
    Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] (ab253238)

    CDNF was immunoprecipitated from 0.35 mg human skeletal muscle tissue lysate with ab253238 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab253238 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/1000 dilution.

    Lane 1: Human skeletal muscle tissue lysate 10 µg (input)
    Lane 2: ab253238 IP in human skeletal muscle tissue lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253238 in human skeletal muscle lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

     

  • Western blot - Anti-CDNF antibody [EPR22334-99] (ab253238)
    Western blot - Anti-CDNF antibody [EPR22334-99] (ab253238)
    All lanes : Anti-CDNF antibody [EPR22334-99] (ab253238) at 1/1000 dilution

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human brain tissue lysate
    Lane 3 : Human kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 21 kDa


    Exposure time: 3 minutes


    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    Kidney and brain are CDNF low expressing tissues (PMID:17611540).

    This blot was developed using a higher sensitivity ECL substrate.

  • Western blot - Anti-CDNF antibody [EPR22334-99] (ab253238)
    Western blot - Anti-CDNF antibody [EPR22334-99] (ab253238)
    All lanes : Anti-CDNF antibody [EPR22334-99] (ab253238) at 1/1000 dilution

    Lane 1 : Mouse skeletal muscle tissue lysate
    Lane 2 : Rat skeletal muscle lysate
    Lane 3 : Human skeletal muscle lysate
    Lane 4 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 3-4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?



    Exposure times: Lanes 1 and 2: 37 seconds; Lanes 3 and 4: 3 minutes.

    Blocking/diluting buffer and concentration: 5% NFDM/TBST 

    The expression profile and molecular weight observed are consistent with literature (PMID: 17611540).

  • Western blot - Anti-CDNF antibody [EPR22334-99] (ab253238)
    Western blot - Anti-CDNF antibody [EPR22334-99] (ab253238)
    Anti-CDNF antibody [EPR22334-99] (ab253238) at 1/1000 dilution + Recombinant Human CDNF Protein (aa25-187) 10 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 21 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Diluting  buffer and concentration: 5% NFDM/TBST 

  • Anti-CDNF antibody [EPR22334-99] (ab253238)
    Anti-CDNF antibody [EPR22334-99] (ab253238)

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