Mouse ARP1 ELISA Kit (ab267590)
Key features and details
- Sensitivity: 0.85 ng/ml
- Range: 0.819 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse ARP1 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.85 ng/ml -
Range
0.819 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 127.4 85% - 145% Plasma 101.3 73% - 125% Cell culture media 132.1 118% - 142% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse ARP1 ELISA Kit is designed for the quantitative determination of ARP1 in cell culture supernatants, plasma and serum samples.
Δ Note: Mouse ARP1 concentration is low in normal serum/plasma and may not be detectable in this assay.
This assay employs an antibody specific for mouse ARP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and ARP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse ARP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ARP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 900X HRP-Streptavidin Concentrate 1 x 200µl Anti-Mouse ARP1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Mouse ARP1 Detection Antibody 2 vials Mouse ARP1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Tissue specificity
Highly expressed in adrenal gland, placenta, thyroid gland, heart, skeletal muscle and small intestine. Weakly expressed in testis, ovary, colon, pancreas, kidney and stomach. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ANG 3
- ANG-3
- ANG3
see all -
Database links
- Entrez Gene: 72713 Mouse
- SwissProt: Q640P2 Mouse
- Unigene: 389206 Mouse
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.