Methylated DNA Immunoprecipitation (MeDIP) Kit - Tissue (ab117136)
Key features and details
- Assay time: 3 hr 30 min
- Sample type: Tissue
Overview
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Product name
Methylated DNA Immunoprecipitation (MeDIP) Kit - Tissue
See all hydroxymethylated DNA kits -
Sample type
Tissue -
Assay time
3h 30m -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Product overview
DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.
Methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117136) enables the user to enrich methylated DNA directly from various mammalian tissues by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 24 tests 48 tests 8-Well Assay Strips (with Frame) 3 units 6 units 8-Well Strip Caps 3 units 6 units Anti-5-methylcytosine (1mg/mL) 1 x 25µl 1 x 50µl Antibody buffer 1 x 15ml 1 x 30ml Binding buffer 1 x 5ml 1 x 8ml ChIP dilution buffer 1 x 4ml 1 x 6ml DNA release buffer 1 x 2ml 2 x 2ml Elution buffer 1 x 0.6ml 1 x 1.2ml F-Collection Tube 30 units 50 units F-Spin Column 30 units 50 units Homogenizing Buffer 1 x 5ml 1 x 10ml Lysis buffer 1 x 4ml 1 x 6ml Normal Mouse IgG (1 mg/mL) 1 x 10µl 1 x 10µl Proteinase K (10 mg/mL) 1 x 25µl 1 x 50µl Reverse buffer 1 x 2ml 2 x 2ml Wash buffer 1 x 28ml 2 x 28ml -
Research areas
