Lysine Assay Kit (Fluorometric) (ab273311)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Cell Lysate, Plasma, Serum, Tissue Homogenate
- Sensitivity: 5 µM
Overview
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Product name
Lysine Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Serum, Plasma, Cell Lysate, Tissue Homogenate -
Assay type
Quantitative -
Sensitivity
5 µM -
Assay duration
Multiple steps standard assay -
Product overview
Lysine Assay Kit (Fluorometric) (ab273311) allows the highly sensitive quantification of L-lysine levels in various biological samples. The assay is based on the selective enzymatic metabolism of lysine, yielding an oxidized intermediate which reacts with a fluorogenic probe to form a stable fluorophore (Ex/Em = 538/587 nm). The assay is not affected by the physiological concentration of other amino acids and is high-throughput adaptable.
The kit can detect less than 5 μM Lysine in samples.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Developer Mix (Lyophilized) 1 vial Lysine Assay Buffer 1 x 25ml Lysine Enzyme Mix (Lyophilized) 1 vial Lysine Probe 1 x 200µl Lysine Standard (Lyophilized) 1 vial
Images
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Specificity for detection of L-Lysine (LYS) over other common amino acids.
At a 10-fold molar excess (10 nmole/well) versus L-Lysine (1 nmole/well), all other amino acids tested contributed ≤10% interference.
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Estimation of total L-Lysine in pooled normal human plasma, single donor off-the-clot human serum, single donor human saliva and cell culture growth medium.Estimation of total L-Lysine in pooled normal human plasma (5 μL/well), single donor off-the-clot human serum (5 μL/well), single donor human saliva (5 μL/well) and cell culture growth medium (DMEM/F12 with 10% FBS, 2 μL/well).
L-Lysine concentrations for plasma, serum and saliva samples were 185.7 ± 24.9 μM, 195.1 ± 28.9 μM and 14.03 ± 1.31 μM, respectively, whereas the concentration for DMEM/F12 culture medium was 518.6 ± 32.4 μM. Data are mean ± SD of at least 3 replicates. Samples were deproteinized using 10 kDa MWCO spin columns and assayed according to the kit protocol.