Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)
![Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)](https://www.abcam.com/ps/products/247/ab247411/Images/ab247411-410615-anti-p53-phospho-s33-antibody-ep2393y-bsa-and-azide-free-western-blot.jpg "Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2393Y] to p53 (phospho S33) - BSA and Azide free
- Suitable for: ICC, Dot blot, WB
- Reacts with: Human
Overview
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Product name
Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free
See all p53 primary antibodies -
Description
Rabbit monoclonal [EP2393Y] to p53 (phospho S33) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody detects p53 phosphorlyated at serine 33.
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Tested applications
Suitable for: ICC, Dot blot, WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247411 is the carrier-free version of ab75867 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247411 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP2393Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)All lanes : Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867) at 1/5000 dilution
Lane 1 : T-47D whole cell lysate - untreated
Lane 2 : T-47D whole cell lysate - treated with Etoposide
Lane 3 : T-47D whole cell lysate - treated with Etoposide followed by Alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 1 minuteThis data was developed using ab75867, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry - Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)](https://www.abcam.com/ps/products/247/ab247411/Images/ab247411-3-anti-p53-phospho-s33-antibody-ep2393y-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)This data was developed using ab75867, the same antibody clone in a different buffer formulation.Immunocytochemistry/immunofluorescence staining of 4% PFA fixed; 0.1% Triton-X permeabilized A431 (human epidermoid carcinoma) cells with ab75867 at dilution of 1/500. The secondary antibody used was Alexa Fluor® 488; goat anti-rabbit IgG (ab150077) at a dilution of 1/1000. Nucleus was counter-stained with DAPI (blue). ab7291, a mouse anti-tubulin antibody (1/500) was used to stain tubulin along with ab150120 (AlexaFluor®594 goat anti-mouse secondary, 1/1000). Confocal image showed increased nuclear staining after ETP (etoposide; 25 μM, 5h) treatment on A431 cells. The LP treatment decreased the increased nuclear staining caused by ETP. ab179477 (1/500) was used as a Pan control for ab75867. The results showed nuclear staining on untreated, ETP and ETP+LP treated Hela cells. -
![Immunocytochemistry - Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)](https://www.abcam.com/ps/products/247/ab247411/Images/ab247411-2-anti-p53-phospho-s33-antibody-ep2393y-bsa-and-azide-free-immunocytochemistry.gif)
Immunocytochemistry - Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411) -
![Dot Blot - Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)](https://www.abcam.com/ps/products/247/ab247411/Images/ab247411-1-anti-p53-phospho-s33-antibody-ep2393y-bsa-and-azide-free-dot-blot.jpg)
Dot Blot - Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)This data was developed using ab75867, the same antibody clone in a different buffer formulation.Dot blot analysis of p53 (phospho S33) peptide (Lane 1) and p53 non-phospho peptide (Lane 2) labelling p53 (phospho S33) with ab75867 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.Blocking and dilution buffer: 5% NFDM/TBST.Exposure time: 3 minutes. -
![Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)](https://www.abcam.com/ps/products/247/ab247411/Images/ab247411-5-benefits-of-recombinant-antibodies.png)
Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)