Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2393Y] to p53 (phospho S33) - BSA and Azide free
  • Suitable for: ICC, Dot blot, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free
    See all p53 primary antibodies

  • Description

    Rabbit monoclonal [EP2393Y] to p53 (phospho S33) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects p53 phosphorlyated at serine 33.

  • Tested applications

    Suitable for: ICC, Dot blot, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247411 is the carrier-free version of ab75867 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247411 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)
    Western blot - Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)
    All lanes : Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867) at 1/5000 dilution

    Lane 1 : T-47D whole cell lysate - untreated
    Lane 2 : T-47D whole cell lysate - treated with Etoposide
    Lane 3 : T-47D whole cell lysate - treated with Etoposide followed by Alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa


    Exposure time: 1 minute


    This data was developed using ab75867, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)
    Immunocytochemistry - Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)
    This data was developed using ab75867, the same antibody clone in a different buffer formulation.Immunocytochemistry/immunofluorescence staining of 4% PFA fixed; 0.1% Triton-X permeabilized A431 (human epidermoid carcinoma) cells with ab75867 at dilution of 1/500. The secondary antibody used was Alexa Fluor® 488; goat anti-rabbit IgG (ab150077) at a dilution of 1/1000. Nucleus was counter-stained with DAPI (blue). ab7291, a mouse anti-tubulin antibody (1/500) was used to stain tubulin along with ab150120 (AlexaFluor®594 goat anti-mouse secondary, 1/1000). Confocal image showed increased nuclear staining after ETP (etoposide; 25 μM, 5h) treatment on A431 cells. The LP treatment decreased the increased nuclear staining caused by ETP. ab179477 (1/500) was used as a Pan control for ab75867. The results showed nuclear staining on untreated, ETP and ETP+LP treated Hela cells.
  • Immunocytochemistry - Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)
    Immunocytochemistry - Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)
    This data was developed using ab75867, the same antibody clone in a different buffer formulation.ab75867 at 1/100 dilution staining phosphorylated p53 in A431 cells by Immunofluorescence.
  • Dot Blot - Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)
    Dot Blot - Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)
    This data was developed using ab75867, the same antibody clone in a different buffer formulation.Dot blot analysis of p53 (phospho S33) peptide (Lane 1) and p53 non-phospho peptide (Lane 2) labelling p53 (phospho S33) with ab75867 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.Blocking and dilution buffer: 5% NFDM/TBST.Exposure time: 3 minutes.
  • Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)
    Anti-p53 (phospho S33) antibody [EP2393Y] - BSA and Azide free (ab247411)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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