Liver Arginase overexpression 293T lysate (whole cell) (ab94083)
Overview
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Product name
Liver Arginase overexpression 293T lysate (whole cell)
See all Liver Arginase lysates -
General notes
ab94083 is a 293T cell transfected lysate in which Human liver Arginase has been transiently over-expressed using a pCMV-ARG1 Full length plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes.
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Pathway: Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. Disease: Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia. Similarity: Belongs to the arginase family.
Images
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SDS-PAGE - Liver Arginase overexpression 293T lysate (whole cell) (ab94083)ab94083 at 15µg/lane on an SDS-PAGE gel.
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Western blot - Liver Arginase overexpression 293T lysate (whole cell) (ab94083)All lanes : Anti-liver Arginase antibody (ab89184) at 1/500 dilution
Lane 1 :Liver Arginase overexpression 293T lysate (whole cell) (ab94083)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
