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Neuroscience Neurotransmission Receptors / Channels Potassium Channels

KChIP2 overexpression 293T lysate (whole cell) (ab94274)

KChIP2 overexpression 293T lysate (whole cell) (ab94274)
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Overview

  • Product name

    KChIP2 overexpression 293T lysate (whole cell)
  • General notes

    ab94274 is a 293T cell transfected lysate in which Human KChIP2 has been transiently over-expressed using a pCMV-KChIP2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes

  • Tested applications

    Suitable for: WBmore details

Properties

  • Mycoplasma free

    Yes
  • Form

    Liquid
  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Potassium Channels
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias
  • Background

    Function: Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Modulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND2/Kv4.2 and KCND3/Kv4.3 currents. Involved in KCND2 and KCND3 trafficking to the cell surface. May be required for the expression of I(To) currents in the heart. PTM: Palmitoylated. Palmitoylation enhances association with the plasma membrane. Similarity: Belongs to the recoverin family. Contains 4 EF-hand domains. Tissue specificity: Expressed in brain. Colocalizes with KCND2 in excitatory neurons including cortical and hippocampal CA1 pyramidal cells. Isoform 3 is expressed in heart and in umbilical vein endothelial cells. Not expressed in fetal heart.

Images

  • SDS-PAGE - KChIP2 overexpression 293T lysate (whole cell) (ab94274)
    SDS-PAGE - KChIP2 overexpression 293T lysate (whole cell) (ab94274)
    ab94274 at 15µg/lane on an SDS-PAGE gel.
  • Western blot - KChIP2 overexpression 293T lysate (whole cell) (ab94274)
    Western blot - KChIP2 overexpression 293T lysate (whole cell) (ab94274)
    All lanes : Anti-KChIP2 antibody (ab57207) at 1/500 dilution

    Lane 1 : KChIP2 overexpression 293T lysate (whole cell) (ab94274)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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