Human XIAP Antibody Pair - BSA and Azide free (ab244196)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human XIAP Antibody Pair - BSA and Azide free
See all XIAP kits -
Assay type
ELISA set -
Range
0.47 ng/ml - 30 ng/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human XIAP. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab234576), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human XIAP Capture Antibody (unconjugated) 1 x 100µg Human XIAP Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. -
Tissue specificity
Ubiquitous, except peripheral blood leukocytes. -
Involvement in disease
Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma. -
Sequence similarities
Belongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 RING-type zinc finger. -
Domain
The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains. -
Post-translational
modificationsUbiquitinated and degraded by the proteasome in apoptotic cells.
Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- AP 13
- API3
- Apoptosis Inhibitor 3
see all -
Database links
- Entrez Gene: 331 Human
- Omim: 300079 Human
- SwissProt: P98170 Human
- Unigene: 356076 Human
Images
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Sandwich ELISA - Human XIAP Antibody Pair - BSA and Azide free (ab244196)To learn more about the advantages of recombinant antibodies see here.