Human Vitronectin/S-Protein Antibody Pair - BSA and Azide free (ab244120)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human Vitronectin/S-Protein Antibody Pair - BSA and Azide free
See all Vitronectin/S-Protein kits -
Assay type
ELISA set -
Range
0.06 ng/ml - 4 ng/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human Vitronectin. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab234577), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human Vitronectin/S-Protein Capture Antibody (unconjugated) 1 x 100µg Human Vitronectin/S-Protein Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. -
Tissue specificity
Plasma. -
Sequence similarities
Contains 4 hemopexin repeats.
Contains 1 SMB (somatomedin-B) domain. -
Domain
The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin. -
Post-translational
modificationsSulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium. -
Cellular localization
Secreted, extracellular space. - Information by UniProt
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Alternative names
- Complement S Protein
- Epibolin
- S Protein
see all -
Database links
- Entrez Gene: 7448 Human
- Omim: 193190 Human
- SwissProt: P04004 Human
- Unigene: 2257 Human
Images
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Sandwich ELISA - Human Vitronectin/S-Protein Antibody Pair - BSA and Azide free (ab244120)To learn more about the advantages of recombinant antibodies see here.