Human TSLP ELISA Kit (ab192149)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TSLP ELISA Kit
See all TSLP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 8 4.4% Inter-assay Sample n Mean SD CV% Overall 3 5% -
Sample type
Cell culture supernatant, Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
3 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 88 83% - 93% Cell culture media 121 114% - 126% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human TSLP ELISA Kit (ab192149) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TSLP protein in cell culture supernatant and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TSLP with 3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Thymic stromal lyphopoietin (TSLP) is a protein belonging to the IL-7 cytokine family. TSLP is released by epithelial cells and keratinocytes in response to trauma, infection or allergen. It acts upon dendritic cells to mediate TH2 mediated immune response and appears to be a key mediator in many inflammatory diseases such as: Asthma, Eczema, and Atopic Dermatitis. TSLP also acts upon mast cells promote the release of IL-5, -6, and -13 in the presence of IL-1 and TNF-α.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human TSLP Capture Antibody 1 x 600µl 10X Human TSLP Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human TSLP Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
The TSLP gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. This mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, chronic obstructive pulmonary disease and allergic inflammation. The protein is therefore considered a potential therapeutic target for the treatment of these diseases. Alternative splicing of this gene results in multiple transcript variants. -
Cellular localization
Secreted -
Alternative names
- Thymic stromal lymphopoietin
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Database links
- Entrez Gene: 85480 Human
- Omim: 607003 Human
- SwissProt: Q969D9 Human
- Unigene: 389874 Human
Images
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Other - Human TSLP ELISA Kit (ab192149)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of TSLP standard curve prepared in Sample Diluent 50BS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of TSLP standard curve prepared in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of normal Human serum (NHS) native and spiked with 0.5 ng/mL recombinant TSLP protein to within the working range of the assay.The samples and standard curve was diluted in Sample Diluent 50BS. Background-subtracted data values (mean +/- SD, n = 2) are graphed.
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Assay for TSLP levels from 10 Individual Donor Human Serum samples and patients clinically diagnosed with Atopic Dermatitis (A.D.) to within the working range of the assay.Severity of A.D. symptoms and medical histories of all donors are unknown. Samples and standard curve was diluted in Sample Diluent 50BS. Background-subtracted data values (mean +/- SD, n = 2) are graphed.
