Human TRIM21 (SS-A) knockout A549 cell pellet (ab279243)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human TRIM21 knockout A549 cell pellet 1 vial Human wild-type A549 cell pellet 1 vial -
Research areas
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Cell type
epithelial -
Disease
Carcinoma -
STR Analysis
Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12
Images
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Western blot - Human TRIM21 (SS-A) knockout A549 cell pellet (ab279243)
Lane 1:Wild-type A549 (Human lung carcinoma cell line) whole cell lysate (20 ug)
Lane 2:TRIM21 knockout A549 (Human lung carcinoma cell line) whole cell lysate (20 ug)
Lane 3:HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
Lane 4:MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (20 ug)ab207728 was shown to specifically react with TRIM21/SS-A in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267024 (knockout cell lysate ab257766) was used. Wild-type and TRIM21/SS-A knockout samples were subjected to SDS-PAGE. ab207728 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human TRIM21 (SS-A) knockout A549 cell pellet (ab279243)Allele-1: 8 bp deletion in exon4
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Sanger Sequencing - Human TRIM21 (SS-A) knockout A549 cell pellet (ab279243)Allele-2: 1 bp deletion in exon4
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Sanger Sequencing - Human TRIM21 (SS-A) knockout A549 cell pellet (ab279243)Allele-3: 1 bp insertion in exon4