Human Transketolase ELISA Kit (TKT) (ab187398)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 800 pg/ml
- Range: 4.68 ng/ml - 300 ng/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Transketolase ELISA Kit (TKT)
See all Transketolase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% HeLa extract 5 2.3% Inter-assay Sample n Mean SD CV% HeLa extract 3 9.2% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
800 pg/ml -
Range
4.68 ng/ml - 300 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 109.5 107.2% - 114% Cell culture media 114 111.9% - 117.7% Goat Serum 74.6 73% - 75.8% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Transketolase ELISA Kit (TKT) (ab187398) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Transketolase (TKT) protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Transketolase (TKT) with 800 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Transketolase is a key enzyme in the non-oxidative branch of the pentose phosphate pathway that transfers a two-carbon glycoaldehyde unit from ketose-donor to aldose-acceptor sugars. The enzyme is also involved in the photosynthetic Calvin cycle in plants and autotrophic bacteria. Thiamine diphosphate and calcium are essential cofactors in Transketolase catalyzed reactions. In mammals, Transketolase connects the pentose phosphate pathway to glycolysis, feeding excess sugar phosphates into the main carbohydrate metabolic pathways. Its presence is necessary for the production of NADPH, especially in tissues actively engaged in biosyntheses, such as fatty acid synthesis by the liver and mammary glands, and for steroid synthesis by the liver and adrenal glands. Transketolase activity is decreased in deficiency of thiamine, which is generally due to malnutrition, i.e. Wernicke-Korsakoff syndrome.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human TKT Capture Antibody 2 x 300µl 10X Human TKT Detector Antibody 2 x 300µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml Human TKT Lyophilized Recombinant Protein 2 vials TMB Development Solution 1 x 12ml -
Research areas
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Cellular localization
Cytosol, nuclear, peroxisome, extracellular vesicular exosome -
Alternative names
- epididymis luminal protein 107
- HEL107
- TK
see all -
Database links
- Entrez Gene: 7086 Human
- Omim: 606781 Human
- SwissProt: P29401 Human
- Unigene: 89643 Human
Images
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Other - Human Transketolase ELISA Kit (TKT) (ab187398)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of TKT standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of cell extracts within the working range of the assay.Background-subtracted data values (mean +/- SD, n = 2) are graphed.
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Titration of Human tissue homogenate extracts within the working range of the assay.Background-subtracted data values (mean +/- SD, n = 2) are graphed.
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TKT concentrations in HepG2 and HeLa cells.Three concentrations (within the working range of the assay) of the cell extracts were analyzed in duplicates with this kit. The concentrations of TKT were interpolated from data values using TKT standard curve and graphed in ng of TKT per mg of extract (mean +/- SD, n=3).