Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human TRAF2 Antibody Pair - BSA and Azide free (ab244032)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

Overview

  • Product name

    Human TRAF2 Antibody Pair - BSA and Azide free
    See all TRAF2 kits

  • Assay type

    ELISA set

  • Range

    1.6 ng/ml - 100 ng/ml

  • Species reactivity

    Reacts with: Human

  • Product overview

    The Antibody Pair can be used to quantify Human TRAF2. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab226893), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human TRAF2 Capture Antibody (unconjugated) 1 x 100µg
    Human TRAF2 Detector Antibody (unconjugated) 1 x 100µg

  • Research areas

  • Function

    Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Belongs to the TNF receptor-associated factor family. A subfamily.
    Contains 1 MATH domain.
    Contains 1 RING-type zinc finger.
    Contains 2 TRAF-type zinc fingers.

  • Domain

    The coiled coil domain mediates homo- and hetero-oligomerization.
    The MATH/TRAF domain binds to receptor cytoplasmic domains.
    The RING-type zinc finger domain is essential for E3 ubiquitin-protein ligase activity. It is not essential for the stabilization of BIRC2, or for the ubiquitination of RIPK1 in response to TNFR1 signaling.

  • Post-translational
    modifications

    Phosphorylated at several serine residues within the first 128 amino acid residues. Phosphorylated at Thr-117 in response to signaling via TNF and TNFRSF1A. Phosphorylation at Thr-117 is required for 'Lys-63'-linked polyubiquitination, but not for 'Lys-48'-linked polyubiquitination. Phosphorylation at Thr-117 is important for interaction with IKKA and IKKB, activation of IKK and subsequent activation of NF-kappa-B.
    Undergoes both 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination. Polyubiquitinated via 'Lys-63'-linked ubiquitin in response to TNF signaling; this requires prior phosphorylation at Thr-117. 'Lys-63'-linked polyubiquitination promotes TRAF2-mediated activation of NF-kappa-B. Can be polyubiquitinated at several Lys residues via 'Lys-48'-linked ubiquitin chains in response to TNF signaling, leading to proteasomal degradation. Autoubiquitinated, leading to its subsequent proteasomal degradation. Polyubiquitinated by BIRC2 and SIAH2, leading to its subsequent proteasomal degradation. Deubiquitinated by CYLD, a protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Alternative names

    • E3 ubiquitin-protein ligase TRAF2
    • MGC:45012
    • OTTHUMP00000022625
    • OTTHUMP00000064745
    • TNF receptor associated factor 2
    • TNF receptor-associated factor 2
    • TNF receptor-associated protein
    • TRAF 2
    • TRAF2
    • TRAF2_HUMAN
    • TRAP
    • TRAP 3
    • TRAP3
    • Tumor necrosis factor type 2 receptor associated protein 3
    • Tumor necrosis factor type 2 receptor-associated protein 3
    see all

  • Database links

    Images

    • Sandwich ELISA - Human TRAF2 Antibody Pair - BSA and Azide free (ab244032)
      Sandwich ELISA - Human TRAF2 Antibody Pair - BSA and Azide free (ab244032)
      To learn more about the advantages of recombinant antibodies see here.

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