Human Toll-like Receptor 9 ELISA Kit (ab252358)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 248 pg/ml
- Range: 1.25 ng/ml - 80 ng/ml
- Sample type: Cell culture extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Toll-like Receptor 9 ELISA Kit
See all TLR9 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% extract 8 1% -
Sample type
Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
248 pg/ml -
Range
1.25 ng/ml - 80 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 115 % - % -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Toll-like Receptor 9 ELISA Kit (ab252358) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Toll-like Receptor 9 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Toll-like Receptor 9 with 248 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Toll-like Receptor 9 Capture Antibody 1 x 600µl 10X Human Toll-like Receptor 9 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human Toll-like Receptor 9 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
Cellular localization
Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. - Information by UniProt
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Alternative names
- CD 289
- CD289
- TLR 9
see all -
Database links
- Entrez Gene: 54106 Human
- Omim: 605474 Human
- SwissProt: Q9NR96 Human
- Unigene: 87968 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Toll-like Receptor 9 standard curve in 1X Cell Extraction Buffer PTR.The Toll-like Receptor 9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Toll-like Receptor 9 in human Daudi cell extract based on a 125 µg/mL extract load.The concentrations of Toll-like Receptor 9 were measured in duplicate and interpolated from the Toll-like Receptor 9 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of spike Toll-like Receptor 9 in human Ramos cell extract based on a 250 µg/mL extract load.The concentrations of Toll-like Receptor 9 were measured in duplicate and interpolated from the Toll-like Receptor 9 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Human Toll-like Receptor 9 ELISA Kit (ab252358)To learn more about the advantages of recombinant antibodies see here.
