Human MIP1b Matched Antibody Pair Kit (ab219534)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 7.81 pg/ml - 500 pg/ml
Overview
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Product name
Human MIP1b Matched Antibody Pair Kit
See all CCL4/MIP-1 beta kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
1.05 pg/ml -
Range
7.81 pg/ml - 500 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human MIP1b Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human MIP1b.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab216173. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human MIP1b Capture Antibody 2 x 50µg 1 x 50µg Human MIP1b Detector Antibody 2 x 12.5µg 1 x 12.5µg Human MIP1b Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- MIP 1 beta
- Secreted protein G 26
- ACT 2
see all -
Database links
- Entrez Gene: 388372 Human
- Entrez Gene: 6351 Human
- Entrez Gene: 9560 Human
- Omim: 182284 Human
- Omim: 610757 Human
- Omim: 603782 Human
- SwissProt: Q8NHW4 Human
- SwissProt: P13236 Human
see all
Images
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Human MIP1b Standard Curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human MIP1b Matched Antibody Pair Kit (ab219534)To learn more about the advantages of recombinant antibodies see here.
