Anti-TRAF1 antibody [1F3] (ab252875)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [1F3] to TRAF1
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-TRAF1 antibody [1F3]
See all TRAF1 primary antibodies -
Description
Rat monoclonal [1F3] to TRAF1 -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
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Positive control
- WB: THP-1 treated with 5ng/ml TNF-alpha for 6h, whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
1F3 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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All lanes : Anti-TRAF1 antibody [1F3] (ab252875) at 1/1000 dilution
Lane 1 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
Lane 2 : THP-1 treated with 5ng/ml TNF-alpha for 6h, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 46 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-TRAF1 antibody [1F3] (ab252875) at 1/1000 dilution
Lane 1 : NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell), whole cell lysate
Lane 2 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate
Lane 3 : Raji (human Burkitt's lymphoma lymphoblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 46 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?The bands observed in lane 1 is consistent with what has been described in the literature (PMID: 11181075).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1-2: 3 mins; Lane 3: 37 secs.
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