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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-TRAF1 antibody [1F3] (ab252875)

Price and availability

307 566 ₸

Availability

Order now and get it on Friday March 19, 2021

Anti-TRAF1 antibody [1F3] (ab252875)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [1F3] to TRAF1
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-TRAF1 antibody [1F3]
    See all TRAF1 primary antibodies
  • Description

    Rat monoclonal [1F3] to TRAF1
  • Host species

    Rat
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • WB: THP-1 treated with 5ng/ml TNF-alpha for 6h, whole cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Clonality

    Monoclonal
  • Clone number

    1F3
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Cancer
    • Growth factors
    • TNF

Images

  • Western blot - Anti-TRAF1 antibody [1F3] (ab252875)
    Western blot - Anti-TRAF1 antibody [1F3] (ab252875)
    All lanes : Anti-TRAF1 antibody [1F3] (ab252875) at 1/1000 dilution

    Lane 1 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
    Lane 2 : THP-1 treated with 5ng/ml TNF-alpha for 6h, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 46 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-TRAF1 antibody [1F3] (ab252875)
    Western blot - Anti-TRAF1 antibody [1F3] (ab252875)
    All lanes : Anti-TRAF1 antibody [1F3] (ab252875) at 1/1000 dilution

    Lane 1 : NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell), whole cell lysate
    Lane 2 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate
    Lane 3 : Raji (human Burkitt's lymphoma lymphoblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 46 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    The bands observed in lane 1 is consistent with what has been described in the literature (PMID: 11181075).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1-2: 3 mins; Lane 3: 37 secs.

  • Anti-TRAF1 antibody [1F3] (ab252875)
    Anti-TRAF1 antibody [1F3] (ab252875)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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